Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study

A sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of diphenhydramine hydrochloride in rabbit plasma was developed and validated. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample...

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Detalles Bibliográficos
Autores principales: Ma Jianshe, Zhang, Meiling, Shentu, Yangping, Zhang, Yuqing, Fan, Xiaofang, Gong, Yongsheng
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
diphenhydramine hydrochloride; liquid chromatography-tandem mass spectrometry; pharmacokinetics; plasm
Etilaminas
Técnicas de Química Analítica
Sangre
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8308
http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_1_19.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8308
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
diphenhydramine hydrochloride; liquid chromatography-tandem mass spectrometry; pharmacokinetics; plasm
Etilaminas
Técnicas de Química Analítica
Sangre
spellingShingle Farmacia
diphenhydramine hydrochloride; liquid chromatography-tandem mass spectrometry; pharmacokinetics; plasm
Etilaminas
Técnicas de Química Analítica
Sangre
Ma Jianshe
Zhang, Meiling
Shentu, Yangping
Zhang, Yuqing
Fan, Xiaofang
Gong, Yongsheng
Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study
topic_facet Farmacia
diphenhydramine hydrochloride; liquid chromatography-tandem mass spectrometry; pharmacokinetics; plasm
Etilaminas
Técnicas de Química Analítica
Sangre
description A sensitive and selective liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of diphenhydramine hydrochloride in rabbit plasma was developed and validated. After addition of triazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm × 50 mm, 3.5 μm) column with acetonitrile-0.1 % formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; multiple reaction monitoring (MRM) mode was used to quantification using target fragment ions m/z 255.8 → 166.6 for diphenhydramine hydrochloride and m/z 342.9 → 308.0 for the IS. Calibration plots were linear over the range of 5- 200 ng/mL for diphenhydramine hydrochloride in rabbit plasma. Lower limit of quantitation (LLOQ) for diphenhydramine hydrochloride was 5 ng/mL. RSD of intra-day and inter-day precisions were both less than 10 %. This developed method is successfully used in pharmacokinetic study of diphenhydramine hydrochloride in rabbit.
format Articulo
Articulo
author Ma Jianshe
Zhang, Meiling
Shentu, Yangping
Zhang, Yuqing
Fan, Xiaofang
Gong, Yongsheng
author_facet Ma Jianshe
Zhang, Meiling
Shentu, Yangping
Zhang, Yuqing
Fan, Xiaofang
Gong, Yongsheng
author_sort Ma Jianshe
title Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study
title_short Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study
title_full Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study
title_fullStr Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study
title_full_unstemmed Determination of diphenhydramine hydrochloride in rabbit plasma by LC-MS/MS and its application to a pharmacokinetic study
title_sort determination of diphenhydramine hydrochloride in rabbit plasma by lc-ms/ms and its application to a pharmacokinetic study
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8308
http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_1_19.pdf
work_keys_str_mv AT majianshe determinationofdiphenhydraminehydrochlorideinrabbitplasmabylcmsmsanditsapplicationtoapharmacokineticstudy
AT zhangmeiling determinationofdiphenhydraminehydrochlorideinrabbitplasmabylcmsmsanditsapplicationtoapharmacokineticstudy
AT shentuyangping determinationofdiphenhydraminehydrochlorideinrabbitplasmabylcmsmsanditsapplicationtoapharmacokineticstudy
AT zhangyuqing determinationofdiphenhydraminehydrochlorideinrabbitplasmabylcmsmsanditsapplicationtoapharmacokineticstudy
AT fanxiaofang determinationofdiphenhydraminehydrochlorideinrabbitplasmabylcmsmsanditsapplicationtoapharmacokineticstudy
AT gongyongsheng determinationofdiphenhydraminehydrochlorideinrabbitplasmabylcmsmsanditsapplicationtoapharmacokineticstudy
bdutipo_str Repositorios
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