Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses

Certain bacteria use a type III secretion system (TTSS) to deliver effector proteins that interfere with cell function into host cells. While transcription of genes encoding TTSS components has been demonstrated, studies to date have failed to identify TTSS effector proteins in Bordetella pertussis....

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Detalles Bibliográficos
Autores principales: Fennelly, Neil K., Sisti, Federico, Higgins, Sarah C., Ross, Pádraig J., Van Der Heide, Han, Mooi, Frits R., Boyd, Aoife, Mills, Kingston H. G.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Exactas
Bordetella pertussis
Enfermedades del Sistema Inmune
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83054
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83054
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella pertussis
Enfermedades del Sistema Inmune
spellingShingle Ciencias Exactas
Bordetella pertussis
Enfermedades del Sistema Inmune
Fennelly, Neil K.
Sisti, Federico
Higgins, Sarah C.
Ross, Pádraig J.
Van Der Heide, Han
Mooi, Frits R.
Boyd, Aoife
Mills, Kingston H. G.
Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
topic_facet Ciencias Exactas
Bordetella pertussis
Enfermedades del Sistema Inmune
description Certain bacteria use a type III secretion system (TTSS) to deliver effector proteins that interfere with cell function into host cells. While transcription of genes encoding TTSS components has been demonstrated, studies to date have failed to identify TTSS effector proteins in Bordetella pertussis. Here we present the first evidence of a functionally active TTSS in B. pertussis. Three known TTSS effectors, Bsp22, BopN, and BopD, were identified as TTSS substrates in B. pertussis 12743. We found expression of Bsp22 in a significant proportion of clinical isolates but not in common laboratory-adapted strains of B. pertussis. We generated a TTSS mutant of B. pertussis 12743 and showed that it induced significantly lower respiratory tract colonization in mice than the wild-type bacteria. Respiratory infection of mice with the mutant bacteria induced significantly greater innate proinflammatory cytokine production in the lungs soon after challenge, and this correlated with significantly higher antigen-specific interleukin-17, gamma interferon, and immunoglobulin G responses later in infection. Our findings suggest that the TTSS subverts innate and adaptive immune responses during infection of the lungs and may be a functionally important virulence factor for B. pertussis infection of humans.
format Articulo
Articulo
author Fennelly, Neil K.
Sisti, Federico
Higgins, Sarah C.
Ross, Pádraig J.
Van Der Heide, Han
Mooi, Frits R.
Boyd, Aoife
Mills, Kingston H. G.
author_facet Fennelly, Neil K.
Sisti, Federico
Higgins, Sarah C.
Ross, Pádraig J.
Van Der Heide, Han
Mooi, Frits R.
Boyd, Aoife
Mills, Kingston H. G.
author_sort Fennelly, Neil K.
title Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
title_short Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
title_full Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
title_fullStr Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
title_full_unstemmed Bordetella pertussis expresses a functional type III secretion system that subverts protective innate and adaptive immune responses
title_sort bordetella pertussis expresses a functional type iii secretion system that subverts protective innate and adaptive immune responses
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/83054
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