The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation

The effect of the rhizobium adhesion protein RapA1 on Rhizobium leguminosarum bv. trifolii adsorption to Trifolium pratense (red clover) roots was investigated. We altered RapA1 production by cloning its encoding gene under the plac promoter into the stable vector pHC60. After introducing this plasm...

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Autores principales: Mongiardini, Elías Javier, Ausmees, Nora, Pérez Giménez, Julieta, Althabegoiti, María Julia, Quelas, Juan Ignacio, López García, Silvina Laura, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2008
Materias:
Ciencias Exactas
Adhesin
Attachment
Biofilm
Nodulation
Rhizobium
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83021
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83021
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Adhesin
Attachment
Biofilm
Nodulation
Rhizobium
spellingShingle Ciencias Exactas
Adhesin
Attachment
Biofilm
Nodulation
Rhizobium
Mongiardini, Elías Javier
Ausmees, Nora
Pérez Giménez, Julieta
Althabegoiti, María Julia
Quelas, Juan Ignacio
López García, Silvina Laura
Lodeiro, Aníbal Roberto
The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation
topic_facet Ciencias Exactas
Adhesin
Attachment
Biofilm
Nodulation
Rhizobium
description The effect of the rhizobium adhesion protein RapA1 on Rhizobium leguminosarum bv. trifolii adsorption to Trifolium pratense (red clover) roots was investigated. We altered RapA1 production by cloning its encoding gene under the plac promoter into the stable vector pHC60. After introducing this plasmid in R. leguminosarum bv. trifolii, three to four times more RapA1 was produced, and two to five times higher adsorption to red clover roots was obtained, as compared with results for the empty vector. Enhanced adsorption was also observed on soybean and alfalfa roots, not related to R. leguminosarum cross inoculation groups. Although the presence of 1 mM Ca2+ during rhizobial growth enhanced adsorption, it was unrelated to RapA1 level. Similar effects were obtained when the same plasmid was introduced in Rhizobium etli for its adsorption to bean roots. Although root colonization by the RapA1-overproducing strain was also higher, nodulation was not enhanced. In addition, in vitro biofilm formation was similar to the wild-type both on polar and on hydrophobic surfaces. These results suggest that RapA1 receptors are present in root but not on inert surfaces, and that the function of this protein is related to rhizosphere colonization.
format Articulo
Articulo
author Mongiardini, Elías Javier
Ausmees, Nora
Pérez Giménez, Julieta
Althabegoiti, María Julia
Quelas, Juan Ignacio
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author_facet Mongiardini, Elías Javier
Ausmees, Nora
Pérez Giménez, Julieta
Althabegoiti, María Julia
Quelas, Juan Ignacio
López García, Silvina Laura
Lodeiro, Aníbal Roberto
author_sort Mongiardini, Elías Javier
title The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation
title_short The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation
title_full The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation
title_fullStr The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation
title_full_unstemmed The rhizobial adhesion protein RapA1 is involved in adsorption of rhizobia to plant roots but not in nodulation
title_sort rhizobial adhesion protein rapa1 is involved in adsorption of rhizobia to plant roots but not in nodulation
publishDate 2008
url http://sedici.unlp.edu.ar/handle/10915/83021
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