Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium

A new spectrophotometric method employing a micellar medium is proposed for the determination of paracetamol in pharmaceuticals. The method is based on the acid hydrolysis of paracetamol to p-aminophenol (PAP), which under acidic conditions reacts with p-dimethylaminocinnamaldehyde (pDAC), producing...

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Detalles Bibliográficos
Autores principales:	Sequinel, Rodrigo, Rufino, José L., Pezza, Helena, Pezza, Leonardo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia microwave; paracetamol; p-dimethylaminocinnamaldehyde; spectrophotometry; surfactant Acetaminofén Técnicas de Química Analítica Hidrólisis Aminofenoles
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8302 http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_1_12.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8302
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia microwave; paracetamol; p-dimethylaminocinnamaldehyde; spectrophotometry; surfactant Acetaminofén Técnicas de Química Analítica Hidrólisis Aminofenoles
spellingShingle	Farmacia microwave; paracetamol; p-dimethylaminocinnamaldehyde; spectrophotometry; surfactant Acetaminofén Técnicas de Química Analítica Hidrólisis Aminofenoles Sequinel, Rodrigo Rufino, José L. Pezza, Helena Pezza, Leonardo Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
topic_facet	Farmacia microwave; paracetamol; p-dimethylaminocinnamaldehyde; spectrophotometry; surfactant Acetaminofén Técnicas de Química Analítica Hidrólisis Aminofenoles
description	A new spectrophotometric method employing a micellar medium is proposed for the determination of paracetamol in pharmaceuticals. The method is based on the acid hydrolysis of paracetamol to p-aminophenol (PAP), which under acidic conditions reacts with p-dimethylaminocinnamaldehyde (pDAC), producing a red compound (λmax = 530 nm). This reaction can be enhanced five-fold in the presence of sodium dodecyl sulfate (SDS). The effects of all the parameters involved in both the hydrolysis step and the derivatization reaction were investigated using experimental design methodologies. The method presented a linear range of 0.2 to 3.9 μg mL–1 and an excellent correlation coefficient (r = 0.9996). The limit of detection was estimated to be 30.0 μg/L. The technique was successfully applied for the determination of paracetamol in commercial brands of pharmaceuticals. No interferences from the excipients commonly used in commercial formulations were observed, and the results obtained compared favorably with measurements made using the United States Pharmacopeia procedure, at a 95 % confidence level.
format	Articulo Articulo
author	Sequinel, Rodrigo Rufino, José L. Pezza, Helena Pezza, Leonardo
author_facet	Sequinel, Rodrigo Rufino, José L. Pezza, Helena Pezza, Leonardo
author_sort	Sequinel, Rodrigo
title	Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
title_short	Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
title_full	Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
title_fullStr	Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
title_full_unstemmed	Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
title_sort	spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8302 http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_1_12.pdf
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bdutipo_str	Repositorios
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Spectrophotometric determination of paracetamol in pharmaceuticals using microwave-assisted hydrolysis and a micellar medium

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