Receptor use by pathogenic arenaviruses

The arenavirus family contains several important human pathogens including Lassa fever virus (LASV), lymphocytic choriomeningitis virus (LCMV) and the New World clade B viruses Junin (JUNV) and Machupo (MACV). Previously, α-dystroglycan (α-DG) was identified as a receptor recognized by LASV and cert...

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Detalles Bibliográficos
Autores principales: Reignier, Therese, Oldenburg, Jill, Noble, Beth, Lamb, Erika, Romanowski, Víctor, Buchmeier, Michael J., Cannon, Paula M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Exactas
Alpha-dystroglycan
Arenaviruses
Cellular receptor
Junin virus
Lassa fever virus
LCMV
Machupo virus
Tacaribe virus
Virus entry
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83017
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83017
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Alpha-dystroglycan
Arenaviruses
Cellular receptor
Junin virus
Lassa fever virus
LCMV
Machupo virus
Tacaribe virus
Virus entry
spellingShingle Ciencias Exactas
Alpha-dystroglycan
Arenaviruses
Cellular receptor
Junin virus
Lassa fever virus
LCMV
Machupo virus
Tacaribe virus
Virus entry
Reignier, Therese
Oldenburg, Jill
Noble, Beth
Lamb, Erika
Romanowski, Víctor
Buchmeier, Michael J.
Cannon, Paula M.
Receptor use by pathogenic arenaviruses
topic_facet Ciencias Exactas
Alpha-dystroglycan
Arenaviruses
Cellular receptor
Junin virus
Lassa fever virus
LCMV
Machupo virus
Tacaribe virus
Virus entry
description The arenavirus family contains several important human pathogens including Lassa fever virus (LASV), lymphocytic choriomeningitis virus (LCMV) and the New World clade B viruses Junin (JUNV) and Machupo (MACV). Previously, α-dystroglycan (α-DG) was identified as a receptor recognized by LASV and certain strains of LCMV. However, other studies have suggested that α-DG is probably not used by the clade B viruses, and the receptor(s) for these pathogens is currently unknown. Using pseudotyped retroviral vectors displaying arenavirus glycoproteins (GPs), we are able to explore the role played by the GP in viral entry in the absence of other viral proteins. By examining the ability of the vectors to transduce DG knockout murine embryonic stem (ES) cells, we have confirmed that LASV has an absolute requirement for α-DG in these cells. However, the LCMV GP can still direct substantial entry into murine ES cells in the absence of α-DG, even when the GP from the clone 13 variant is used that has previously been reported to be highly dependent on α-DG for entry. We also found that neither LASV or LCMV pseudotyped vectors were able to transduce human or murine lymphocytes, presumably due to the glycosylation state of α-DG in these cells. In contrast, the JUNV and MACV GPs displayed broad tropism on human, murine and avian cell types, including lymphocytes, and showed no requirement for α-DG in murine ES cells. These findings highlight the importance of molecules other than α-DG for arenavirus entry. An alternate receptor is present on murine ES cells that can be used by LCMV but not by LASV, and which is not available on human or murine lymphocytes, while a distinct and widely expressed receptor(s) is used by the clade B viruses.
format Articulo
Articulo
author Reignier, Therese
Oldenburg, Jill
Noble, Beth
Lamb, Erika
Romanowski, Víctor
Buchmeier, Michael J.
Cannon, Paula M.
author_facet Reignier, Therese
Oldenburg, Jill
Noble, Beth
Lamb, Erika
Romanowski, Víctor
Buchmeier, Michael J.
Cannon, Paula M.
author_sort Reignier, Therese
title Receptor use by pathogenic arenaviruses
title_short Receptor use by pathogenic arenaviruses
title_full Receptor use by pathogenic arenaviruses
title_fullStr Receptor use by pathogenic arenaviruses
title_full_unstemmed Receptor use by pathogenic arenaviruses
title_sort receptor use by pathogenic arenaviruses
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83017
work_keys_str_mv AT reigniertherese receptorusebypathogenicarenaviruses
AT oldenburgjill receptorusebypathogenicarenaviruses
AT noblebeth receptorusebypathogenicarenaviruses
AT lamberika receptorusebypathogenicarenaviruses
AT romanowskivictor receptorusebypathogenicarenaviruses
AT buchmeiermichaelj receptorusebypathogenicarenaviruses
AT cannonpaulam receptorusebypathogenicarenaviruses
bdutipo_str Repositorios
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