Characterization of liposomes coated with S-layer proteins from lactobacilli
The stability of liposomes coated with S-layer proteins from Lactobacillus brevis and Lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. The interactions of the different S-layer proteins with positively charged liposomes prepared wi...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2007
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83016 |
| Aporte de: |
| id |
I19-R120-10915-83016 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Bile and pancreatic bile effect CF and calcein release Lactobacilli Liposome pH stress S-layer protein Thermal shock |
| spellingShingle |
Bioquímica Bile and pancreatic bile effect CF and calcein release Lactobacilli Liposome pH stress S-layer protein Thermal shock Hollmann, A. Delfederico, L. Glikmann, G. De Antoni, Graciela Liliana Semorile, Liliana Carmen Disalvo, E. A. Characterization of liposomes coated with S-layer proteins from lactobacilli |
| topic_facet |
Bioquímica Bile and pancreatic bile effect CF and calcein release Lactobacilli Liposome pH stress S-layer protein Thermal shock |
| description |
The stability of liposomes coated with S-layer proteins from Lactobacillus brevis and Lactobacillus kefir was analyzed as a previous stage to the development of a vaccine vehicle for oral administration. The interactions of the different S-layer proteins with positively charged liposomes prepared with soybean lecithin or dipalmitoylphosphatidylcholine were studied by means of the variation of the Z potential at different protein-lipid ratios, showing that both proteins were able to attach in a greater extent to the surface of soybean lecithin liposomes. The capacity of these particles to retain carboxyfluorescein or calcein by exposure to bile salts, pancreatic extract, pH change and after a thermal shock showed that both S-layer proteins increased the stability of the liposomes in the same magnitude. The non-glycosylated protein from L. brevis protects more efficiently the liposomes at pH 7 than those from L. kefir even without treatment with glutaraldehyde. |
| format |
Articulo Articulo |
| author |
Hollmann, A. Delfederico, L. Glikmann, G. De Antoni, Graciela Liliana Semorile, Liliana Carmen Disalvo, E. A. |
| author_facet |
Hollmann, A. Delfederico, L. Glikmann, G. De Antoni, Graciela Liliana Semorile, Liliana Carmen Disalvo, E. A. |
| author_sort |
Hollmann, A. |
| title |
Characterization of liposomes coated with S-layer proteins from lactobacilli |
| title_short |
Characterization of liposomes coated with S-layer proteins from lactobacilli |
| title_full |
Characterization of liposomes coated with S-layer proteins from lactobacilli |
| title_fullStr |
Characterization of liposomes coated with S-layer proteins from lactobacilli |
| title_full_unstemmed |
Characterization of liposomes coated with S-layer proteins from lactobacilli |
| title_sort |
characterization of liposomes coated with s-layer proteins from lactobacilli |
| publishDate |
2007 |
| url |
http://sedici.unlp.edu.ar/handle/10915/83016 |
| work_keys_str_mv |
AT hollmanna characterizationofliposomescoatedwithslayerproteinsfromlactobacilli AT delfedericol characterizationofliposomescoatedwithslayerproteinsfromlactobacilli AT glikmanng characterizationofliposomescoatedwithslayerproteinsfromlactobacilli AT deantonigracielaliliana characterizationofliposomescoatedwithslayerproteinsfromlactobacilli AT semorilelilianacarmen characterizationofliposomescoatedwithslayerproteinsfromlactobacilli AT disalvoea characterizationofliposomescoatedwithslayerproteinsfromlactobacilli |
| bdutipo_str |
Repositorios |
| _version_ |
1764820488200126464 |