The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies

We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor-Like Protein1 (VSR-1/ATELP1) receptor during the formation of protein storage vacuoles in <i>Arabidopsis thaliana</i> embryos by means...

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Autores principales: Otegui, Marisa, Herder, Rachel, Schulze, Jan, Jung, Rudolf, Staehelin, L. Andrew
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83003
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83003
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
spellingShingle Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
Otegui, Marisa
Herder, Rachel
Schulze, Jan
Jung, Rudolf
Staehelin, L. Andrew
The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
topic_facet Ciencias Naturales
storage proteins
Arabidopsis thaliana embryos
description We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor-Like Protein1 (VSR-1/ATELP1) receptor during the formation of protein storage vacuoles in <i>Arabidopsis thaliana</i> embryos by means of high-pressure freezing/freeze substitution, electron tomography, immunolabeling techniques, and subcellular fractionation. The storage proteins and their processing proteases are segregated from each other within the Golgi cisternae and packaged into separate vesicles. The storage protein-containing vesicles but not the processing enzyme-containing vesicles carry the VSR-1/ATELP1 receptor. Both types of secretory vesicles appear to fuse into a type of prevacuolar multivesicular body (MVB). We have also determined that the proteolytic processing of the 2S albumins starts in the MVBs. We hypothesize that the compartmentalized processing of storage proteins in the MVBs may allow for the sequential activation of processing proteases as the MVB lumen gradually acidifies.
format Articulo
Articulo
author Otegui, Marisa
Herder, Rachel
Schulze, Jan
Jung, Rudolf
Staehelin, L. Andrew
author_facet Otegui, Marisa
Herder, Rachel
Schulze, Jan
Jung, Rudolf
Staehelin, L. Andrew
author_sort Otegui, Marisa
title The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_short The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_full The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_fullStr The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_full_unstemmed The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies
title_sort proteolytic processing of seed storage proteins in arabidopsis embryo cells starts in the multivesicular bodies
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83003
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