Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction

Insulin-like growth factor-I (IGF-I) is emerging as a powerful neuroprotective molecule that is strongly induced in the central nervous system after different insults. We constructed a recombinant adenoviral vector (RAd-IGFI) harboring the gene for rat IGF-I and used it to implement IGF-I gene thera...

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Autores principales: Hereñú, Claudia Beatriz, Cristina, Silvia Carolina, Rimoldi, Omar Jorge, Becú de Villalobos, Damasia, Cambiaggi, Vanina Laura, Portiansky, Enrique Leo, Goya, Rodolfo Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Ciencias Médicas
Ciencias Veterinarias
aging
DA neurodegeneration
TIDA neurons
hyperprolactinemia
IGF-I
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82997
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82997
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Ciencias Veterinarias
aging
DA neurodegeneration
TIDA neurons
hyperprolactinemia
IGF-I
spellingShingle Ciencias Médicas
Ciencias Veterinarias
aging
DA neurodegeneration
TIDA neurons
hyperprolactinemia
IGF-I
Hereñú, Claudia Beatriz
Cristina, Silvia Carolina
Rimoldi, Omar Jorge
Becú de Villalobos, Damasia
Cambiaggi, Vanina Laura
Portiansky, Enrique Leo
Goya, Rodolfo Gustavo
Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
topic_facet Ciencias Médicas
Ciencias Veterinarias
aging
DA neurodegeneration
TIDA neurons
hyperprolactinemia
IGF-I
description Insulin-like growth factor-I (IGF-I) is emerging as a powerful neuroprotective molecule that is strongly induced in the central nervous system after different insults. We constructed a recombinant adenoviral vector (RAd-IGFI) harboring the gene for rat IGF-I and used it to implement IGF-I gene therapy in the hypothalamus of senile female rats, which display hypothalamic dopaminergic (DA) neurodegeneration and as a consequence, chronic hyperprolactinemia. Restorative IGF-I gene therapy was implemented in young (5 months) and senile (28 months) female rats, which received a single intrahypothalamic injection of 3 × 10<SUP>9</SUP> plaque-forming units of RAd-βgal (a control adenoviral vector expressing β-galactosidase) or RAd-IGFI and were killed 17 days post-injection. In the young animals, neither vector modified serum prolactin levels, but in the RAd-IGFI-injected senile rats a nearly full reversion of their hyperprolactinemic status was recorded. Morphometric analysis revealed a significant increase in the total number of tyrosine hydroxylase-positive cells in the hypothalamus of experimental as compared with control senile animals (5874±486 and 3390±498, respectively). Our results indicate that IGF-I gene therapy in senile female rats is highly effective for restoring their hypothalamic DA dysfunction and thus reversing their chronic hyperprolactinemia.
format Articulo
Articulo
author Hereñú, Claudia Beatriz
Cristina, Silvia Carolina
Rimoldi, Omar Jorge
Becú de Villalobos, Damasia
Cambiaggi, Vanina Laura
Portiansky, Enrique Leo
Goya, Rodolfo Gustavo
author_facet Hereñú, Claudia Beatriz
Cristina, Silvia Carolina
Rimoldi, Omar Jorge
Becú de Villalobos, Damasia
Cambiaggi, Vanina Laura
Portiansky, Enrique Leo
Goya, Rodolfo Gustavo
author_sort Hereñú, Claudia Beatriz
title Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
title_short Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
title_full Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
title_fullStr Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
title_full_unstemmed Restorative effect of insulin-like growth factor-I gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
title_sort restorative effect of insulin-like growth factor-i gene therapy in the hypothalamus of senile rats with dopaminergic dysfunction
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/82997
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