Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells

Attachment to epithelial cells in the respiratory tract is a key event in Bordetella pertussis colonization. Filamentous haemagglutinin (FHA) is an important virulence factor mediating adhesion to host cells. In this study, the relevance of the interaction between FHA and adenylate cyclase toxin (AC...

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Autores principales: Pérez Vidakovics, María Laura Anabella, Lamberti, Yanina Andrea, Pol, W.-Ludo van der, Yantorno, Osvaldo Miguel, Rodríguez, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Médicas
Adenylate cyclase
Bacterial attachment
Bordetella pertussis
Filamentous haemagglutinin
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82986
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82986
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Adenylate cyclase
Bacterial attachment
Bordetella pertussis
Filamentous haemagglutinin
spellingShingle Ciencias Médicas
Adenylate cyclase
Bacterial attachment
Bordetella pertussis
Filamentous haemagglutinin
Pérez Vidakovics, María Laura Anabella
Lamberti, Yanina Andrea
Pol, W.-Ludo van der
Yantorno, Osvaldo Miguel
Rodríguez, María Eugenia
Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells
topic_facet Ciencias Médicas
Adenylate cyclase
Bacterial attachment
Bordetella pertussis
Filamentous haemagglutinin
description Attachment to epithelial cells in the respiratory tract is a key event in Bordetella pertussis colonization. Filamentous haemagglutinin (FHA) is an important virulence factor mediating adhesion to host cells. In this study, the relevance of the interaction between FHA and adenylate cyclase toxin (ACT) during bacterial attachment was investigated. Mutants lacking either FHA or ACT showed significantly decreased adherence to epithelial respiratory cells. The use of several ACT-specific monoclonal antibodies and antiserum showed that the decrease in attachment of strains lacking ACT expression could not be explained by the adhesin-like activity of ACT, or a change of any of the biological activities of ACT. Immunoblot analysis showed that the lack of ACT expression did not interfere with FHA localization. An heparin-inhibitable carbohydrate-binding site is crucial in the process of FHA-mediated bacterial binding to epithelial cells. In the presence of heparin attachment of wild-type B. pertussis, but not of the isogenic ACT defective mutant, to epithelial cells was significantly decreased. These results suggest that ACT enhances the adhesive functions of FHA, and modifies the performance of the FHA heparin-inhibitable carbohydrate binding site. We propose that the presence of ACT in the outer membrane of B. pertussis to play a role in the functionality of FHA.
format Articulo
Articulo
author Pérez Vidakovics, María Laura Anabella
Lamberti, Yanina Andrea
Pol, W.-Ludo van der
Yantorno, Osvaldo Miguel
Rodríguez, María Eugenia
author_facet Pérez Vidakovics, María Laura Anabella
Lamberti, Yanina Andrea
Pol, W.-Ludo van der
Yantorno, Osvaldo Miguel
Rodríguez, María Eugenia
author_sort Pérez Vidakovics, María Laura Anabella
title Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells
title_short Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells
title_full Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells
title_fullStr Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells
title_full_unstemmed Adenylate cyclase influences filamentous haemagglutinin-mediated attachment of Bordetella pertussis to epithelial alveolar cells
title_sort adenylate cyclase influences filamentous haemagglutinin-mediated attachment of bordetella pertussis to epithelial alveolar cells
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/82986
work_keys_str_mv AT perezvidakovicsmarialauraanabella adenylatecyclaseinfluencesfilamentoushaemagglutininmediatedattachmentofbordetellapertussistoepithelialalveolarcells
AT lambertiyaninaandrea adenylatecyclaseinfluencesfilamentoushaemagglutininmediatedattachmentofbordetellapertussistoepithelialalveolarcells
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bdutipo_str Repositorios
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