Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria

Rat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically p...

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Detalles Bibliográficos
Autores principales: Gómez Alvis, Alicia, Milesi, María Verónica, Rebolledo, Alejandro, Raingo, Jesica, Grassi de Gende, Ángela Ofelia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82983
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82983
record_format dspace
spelling I19-R120-10915-829832024-06-14T18:46:26Z http://sedici.unlp.edu.ar/handle/10915/82983 Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria Gómez Alvis, Alicia Milesi, María Verónica Rebolledo, Alejandro Raingo, Jesica Grassi de Gende, Ángela Ofelia 2001 2019-10-09T18:09:13Z en Ciencias Exactas Acid pH Capsaicin CGRP receptor blockage Rat atria Ruthenium red Rat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically paced at 2.77 Hz were exposed to a 10-minute period of metabolic acidosis (pH=6.73±0.01, n=28) after: 1) CGRP release induced by capsaicin 0.5 μM; 2) blockage of CGRP release with ruthenium red (RR) 5 μM; 3) no pretreatment; and 4) CGRP receptor blockage with CGRP<SUB>8-37</SUB> 1 μM. Contractility and relaxation were significantly less depressed by acid pH when CGRP release was prevented by RR or CGRP receptor activation was blocked by CGRP<SUB>8-37</SUB>. The results suggest that CGRP release and the activation of CGRP receptors may be physiologically involved in contributing to the depression of contractility and relaxation induced by acid pH in rat atria. Facultad de Ciencias Exactas Articulo Articulo http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf 507-517
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
spellingShingle Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
Gómez Alvis, Alicia
Milesi, María Verónica
Rebolledo, Alejandro
Raingo, Jesica
Grassi de Gende, Ángela Ofelia
Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
topic_facet Ciencias Exactas
Acid pH
Capsaicin
CGRP receptor blockage
Rat atria
Ruthenium red
description Rat atria is richly innervated by sensory nerve fibers that release CGRP when stimulated either by capsaicin or acid pH. We studied the physiological relevance of acid pH-induced CGRP release on changes in atrial contractility and relaxation produced by lowering the pH. Isolated atria electrically paced at 2.77 Hz were exposed to a 10-minute period of metabolic acidosis (pH=6.73±0.01, n=28) after: 1) CGRP release induced by capsaicin 0.5 μM; 2) blockage of CGRP release with ruthenium red (RR) 5 μM; 3) no pretreatment; and 4) CGRP receptor blockage with CGRP<SUB>8-37</SUB> 1 μM. Contractility and relaxation were significantly less depressed by acid pH when CGRP release was prevented by RR or CGRP receptor activation was blocked by CGRP<SUB>8-37</SUB>. The results suggest that CGRP release and the activation of CGRP receptors may be physiologically involved in contributing to the depression of contractility and relaxation induced by acid pH in rat atria.
format Articulo
Articulo
author Gómez Alvis, Alicia
Milesi, María Verónica
Rebolledo, Alejandro
Raingo, Jesica
Grassi de Gende, Ángela Ofelia
author_facet Gómez Alvis, Alicia
Milesi, María Verónica
Rebolledo, Alejandro
Raingo, Jesica
Grassi de Gende, Ángela Ofelia
author_sort Gómez Alvis, Alicia
title Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_short Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_full Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_fullStr Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_full_unstemmed Influence of calcitonin gene-related peptide release on pH-induced mechanical depression in rat atria
title_sort influence of calcitonin gene-related peptide release on ph-induced mechanical depression in rat atria
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/82983
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