The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5

In yeast and mammals, the AAA ATPase Vps4p/SKD1 (for Vacuolar protein sorting 4/SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) is required for the endosomal sorting of secretory and endocytic cargo. We identified a VPS4/SKD1 homolog in Arabidopsis thaliana, which localizes to the cytoplasm and to multiv...

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Autores principales: Haas, Thomas J., Sliwinski, Marek K., Martínez, Dana Ethel, Preuss, Mary, Ebine, Kazuo, Ueda, Takashi, Nielsen, Erik, Odorizzi, Greg, Otegui, Marisa S.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Ciencias Agrarias
ATPasas Tipo P
Arabidopsis
Vacuolas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82962
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82962
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
ATPasas Tipo P
Arabidopsis
Vacuolas
spellingShingle Ciencias Agrarias
ATPasas Tipo P
Arabidopsis
Vacuolas
Haas, Thomas J.
Sliwinski, Marek K.
Martínez, Dana Ethel
Preuss, Mary
Ebine, Kazuo
Ueda, Takashi
Nielsen, Erik
Odorizzi, Greg
Otegui, Marisa S.
The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5
topic_facet Ciencias Agrarias
ATPasas Tipo P
Arabidopsis
Vacuolas
description In yeast and mammals, the AAA ATPase Vps4p/SKD1 (for Vacuolar protein sorting 4/SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) is required for the endosomal sorting of secretory and endocytic cargo. We identified a VPS4/SKD1 homolog in Arabidopsis thaliana, which localizes to the cytoplasm and to multivesicular endosomes. In addition, green fluorescent protein-SKD1 colocalizes on multivesicular bodies with fluorescent fusion protein endosomal Rab GTPases, such as ARA6/RabF1, RHA1/RabF2a, and ARA7/RabF2b, and with the endocytic marker FM4-64. The expression of SKD1E232Q, an ATPase-deficient version of SKD1, induces alterations in the endosomal system of tobacco (Nicotiana tabacum) Bright Yellow 2 cells and ultimately leads to cell death. The inducible expression ofSKD1E232Q in Arabidopsis resulted in enlarged endosomes with a reduced number of internal vesicles. In a yeast two-hybrid screen using Arabidopsis SKD1 as bait, we isolated a putative homolog of mammalian LYST-INTERACTING PROTEIN5 (LIP5)/SKD1 BINDING PROTEIN1 and yeast Vta1p (for Vps twenty associated 1 protein). Arabidopsis LIP5 acts as a positive regulator of SKD1 by increasing fourfold to fivefold its in vitro ATPase activity. We isolated a knockout homozygous Arabidopsis mutant line with a T-DNA insertion in LIP5. lip5 plants are viable and show no phenotypic alterations under normal growth conditions, suggesting that basal SKD1 ATPase activity is sufficient for plant development and growth.
format Articulo
Articulo
author Haas, Thomas J.
Sliwinski, Marek K.
Martínez, Dana Ethel
Preuss, Mary
Ebine, Kazuo
Ueda, Takashi
Nielsen, Erik
Odorizzi, Greg
Otegui, Marisa S.
author_facet Haas, Thomas J.
Sliwinski, Marek K.
Martínez, Dana Ethel
Preuss, Mary
Ebine, Kazuo
Ueda, Takashi
Nielsen, Erik
Odorizzi, Greg
Otegui, Marisa S.
author_sort Haas, Thomas J.
title The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5
title_short The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5
title_full The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5
title_fullStr The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5
title_full_unstemmed The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LYST-INTERACTING PROTEIN5
title_sort arabidopsis aaa atpase skd1 is involved in multivesicular endosome function and interacts with its positive regulator lyst-interacting protein5
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/82962
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