Metformin reactivity with biuret: application to tablets

A spectrophotometric method was developed for the analysis of metformin, based on its reaction with the biuret reagent. A complex with λ<sub>max</sub> = 495 nm and stoichiometry 2:1 (metformin-Cu) is generated wich allows the quantification of metformin in tablets. Physicochemical parame...

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Detalles Bibliográficos
Autores principales:	Chahin, Cecilia L., Delfino, Mario R., Sarno, María del C.
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia biuret; metformin; UV-visible spectrophotometry Biguanidas Técnicas de Química Analítica
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8290 http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_2_9.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8290
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia biuret; metformin; UV-visible spectrophotometry Biguanidas Técnicas de Química Analítica
spellingShingle	Farmacia biuret; metformin; UV-visible spectrophotometry Biguanidas Técnicas de Química Analítica Chahin, Cecilia L. Delfino, Mario R. Sarno, María del C. Metformin reactivity with biuret: application to tablets
topic_facet	Farmacia biuret; metformin; UV-visible spectrophotometry Biguanidas Técnicas de Química Analítica
description	A spectrophotometric method was developed for the analysis of metformin, based on its reaction with the biuret reagent. A complex with λ<sub>max</sub> = 495 nm and stoichiometry 2:1 (metformin-Cu) is generated wich allows the quantification of metformin in tablets. Physicochemical parameters of the product were determined. Sample conditioning by centrifugation was necessary to separate insoluble excipients. Polyvinylpyrrolidone (PVP) was isolated by a solid-liquid extraction with SPE-SCX resin in order to eliminate its interference on the absorbance of the complex. The proposed method was found to be highly precise, having a relative standard deviation, CV % = 0.68. Using this approach the calibration curve showed r<sup>2</sup> = 0.9949 with 95 % confidence level, value included inside the limits established by USP. Accuracy based on the average recovery of known amounts of drug in placebo was in the range of 98.61 to 99.97. Results allow the application of this analytical methodology to metformin tablets.
format	Articulo Comunicacion
author	Chahin, Cecilia L. Delfino, Mario R. Sarno, María del C.
author_facet	Chahin, Cecilia L. Delfino, Mario R. Sarno, María del C.
author_sort	Chahin, Cecilia L.
title	Metformin reactivity with biuret: application to tablets
title_short	Metformin reactivity with biuret: application to tablets
title_full	Metformin reactivity with biuret: application to tablets
title_fullStr	Metformin reactivity with biuret: application to tablets
title_full_unstemmed	Metformin reactivity with biuret: application to tablets
title_sort	metformin reactivity with biuret: application to tablets
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8290 http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_2_9.pdf
work_keys_str_mv	AT chahincecilial metforminreactivitywithbiuretapplicationtotablets AT delfinomarior metforminreactivitywithbiuretapplicationtotablets AT sarnomariadelc metforminreactivitywithbiuretapplicationtotablets
bdutipo_str	Repositorios
_version_	1764820488744337408

Metformin reactivity with biuret: application to tablets

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