Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS

Raman spectroscopy in combination with partial least-squares (PLS) regression method was developed for sulphamethoxazole (SMZ) and trimethoprim (TMP) simultaneous determination in powder mixtures. The calibration set was designed with 18 samples and validation set with 9 samples, containing differen...

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Detalles Bibliográficos
Autores principales:	Silva, Fabiana E. B. da, Ziech, Cristiane, Dias, Yara P., Moreira, Eduardo C., Flores, Érico M. M., Ferrão, Marco F.
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia raman spectroscopy; multivariate calibration; partial least squares regression; sulphamethoxazole; trimethoprim Sulfonamidas Pirimidinas Análisis Cuantitativo Polvos
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8284 http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_2_3.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8284
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia raman spectroscopy; multivariate calibration; partial least squares regression; sulphamethoxazole; trimethoprim Sulfonamidas Pirimidinas Análisis Cuantitativo Polvos
spellingShingle	Farmacia raman spectroscopy; multivariate calibration; partial least squares regression; sulphamethoxazole; trimethoprim Sulfonamidas Pirimidinas Análisis Cuantitativo Polvos Silva, Fabiana E. B. da Ziech, Cristiane Dias, Yara P. Moreira, Eduardo C. Flores, Érico M. M. Ferrão, Marco F. Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS
topic_facet	Farmacia raman spectroscopy; multivariate calibration; partial least squares regression; sulphamethoxazole; trimethoprim Sulfonamidas Pirimidinas Análisis Cuantitativo Polvos
description	Raman spectroscopy in combination with partial least-squares (PLS) regression method was developed for sulphamethoxazole (SMZ) and trimethoprim (TMP) simultaneous determination in powder mixtures. The calibration set was designed with 18 samples and validation set with 9 samples, containing different SMZ and TMP concentrations. The concentration ranges were 400-900 mg/g for SMZ and 80- 240 mg/g for TMP. The proposed procedure was validated by comparison with the official method (HPLC). Mean root square error of calibration (RMSEC) and mean root square error of prediction (RMSEP) were calculated. RMSEC found was 30.96 mg/g to SMZ and 14.36 mg/g to TMP, respectively. RMSEP found was 25.60 mg/g to SMZ and 14.36 mg/g to TMP, respectively. Correlation coefficient (R2) was 0.99 for SMZ and 0.99 for TMP. This parameter evidences a very good agreement between estimated and real values. The results showed that PLS regression model combined with Raman spectroscopy provides a sensitive, fast and simple method for the quantitative analysis of SMZ and TMP mixtures in powder quality control.
format	Articulo Comunicacion
author	Silva, Fabiana E. B. da Ziech, Cristiane Dias, Yara P. Moreira, Eduardo C. Flores, Érico M. M. Ferrão, Marco F.
author_facet	Silva, Fabiana E. B. da Ziech, Cristiane Dias, Yara P. Moreira, Eduardo C. Flores, Érico M. M. Ferrão, Marco F.
author_sort	Silva, Fabiana E. B. da
title	Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS
title_short	Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS
title_full	Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS
title_fullStr	Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS
title_full_unstemmed	Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS
title_sort	quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and pls
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8284 http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_2_3.pdf
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bdutipo_str	Repositorios
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Quantitative determination of sulphamethoxazole and trimethoprim in powder mixtures using raman spectroscopy and PLS

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