Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer

In the course of breast cancer global gene expression studies, we identified an uncharacterized gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly over-expressed in primary tumors from patients with recurrent disease. In this study, we identified RHBDD2 mRNA and protein expression si...

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Autores principales: Abba, Martín Carlos, Lacunza, Ezequiel, Nunez, María I., Colussi, Andrea G., Isla Larrain, Marina Teresita, Segal-Eiras, Amada, Croce, María Virginia, Aldaz, C. Marcelo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Médicas
Breast cancer
Gene amplification
Gene expression profile
RHBDD2
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82772
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82772
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Breast cancer
Gene amplification
Gene expression profile
RHBDD2
spellingShingle Ciencias Médicas
Breast cancer
Gene amplification
Gene expression profile
RHBDD2
Abba, Martín Carlos
Lacunza, Ezequiel
Nunez, María I.
Colussi, Andrea G.
Isla Larrain, Marina Teresita
Segal-Eiras, Amada
Croce, María Virginia
Aldaz, C. Marcelo
Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
topic_facet Ciencias Médicas
Breast cancer
Gene amplification
Gene expression profile
RHBDD2
description In the course of breast cancer global gene expression studies, we identified an uncharacterized gene known as RHBDD2 (Rhomboid domain containing 2) to be markedly over-expressed in primary tumors from patients with recurrent disease. In this study, we identified RHBDD2 mRNA and protein expression significantly elevated in breast carcinomas compared with normal breast samples as analyzed by SAGE (n = 46) and immunohistochemistry (n = 213). Interestingly, specimens displaying RHBDD2 over-expression were predominantly advanced stage III breast carcinomas (p = 0.001). Western-blot, RT-PCR and cDNA sequencing analyses allowed us to identify two RHBDD2 alternatively spliced mRNA isoforms expressed in breast cancer cell lines. We further investigated the occurrence and frequency of gene amplification and over-expression affecting RHBDD2 in 131 breast samples. RHBDD2 gene amplification was detected in 21% of 98 invasive breast carcinomas analyzed. However, no RHBDD2 amplification was detected in normal breast tissues (n = 17) or breast benign lesions (n = 16) (p = 0.014). Interestingly, siRNA-mediated silencing of RHBDD2 expression results in a decrease of MCF7 breast cancer cells proliferation compared with the corresponding controls (p = 0.001). In addition, analysis of publicly available gene expression data showed a strong association between high RHBDD2 expression and decreased overall survival (p = 0.0023), relapse-free survival (p = 0.0013), and metastasis-free interval (p = 0.006) in patients with primary ER-negative breast carcinomas. In conclusion, our findings suggest that RHBDD2 over-expression behaves as an indicator of poor prognosis and may play a role facilitating breast cancer progression.
format Articulo
Articulo
author Abba, Martín Carlos
Lacunza, Ezequiel
Nunez, María I.
Colussi, Andrea G.
Isla Larrain, Marina Teresita
Segal-Eiras, Amada
Croce, María Virginia
Aldaz, C. Marcelo
author_facet Abba, Martín Carlos
Lacunza, Ezequiel
Nunez, María I.
Colussi, Andrea G.
Isla Larrain, Marina Teresita
Segal-Eiras, Amada
Croce, María Virginia
Aldaz, C. Marcelo
author_sort Abba, Martín Carlos
title Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
title_short Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
title_full Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
title_fullStr Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
title_full_unstemmed Rhomboid domain containing 2 (RHBDD2): A novel cancer-related gene over-expressed in breast cancer
title_sort rhomboid domain containing 2 (rhbdd2): a novel cancer-related gene over-expressed in breast cancer
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82772
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