Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis

Multicellular organs are composed of distinct cell types with unique assemblages of translated mRNAs. Here, ribosome-associated mRNAs were immunopurified from specific cell populations of intact seedlings using Arabidopsis thaliana lines expressing a FLAG-epitope tagged ribosomal protein L18 (FLAG-R...

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Detalles Bibliográficos
Autores principales: Mustroph, Angelika, Zanetti, María Eugenia, Jang, Charles J.H., Holtan, Hans E., Repetti, Peter P., Galbraith, David W., Girke, Thomas, Bailey-Serres, Julia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Biología
mRNAs
Arabidopsis
ribosome-associated
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82755
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82755
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
mRNAs
Arabidopsis
ribosome-associated
spellingShingle Biología
mRNAs
Arabidopsis
ribosome-associated
Mustroph, Angelika
Zanetti, María Eugenia
Jang, Charles J.H.
Holtan, Hans E.
Repetti, Peter P.
Galbraith, David W.
Girke, Thomas
Bailey-Serres, Julia
Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis
topic_facet Biología
mRNAs
Arabidopsis
ribosome-associated
description Multicellular organs are composed of distinct cell types with unique assemblages of translated mRNAs. Here, ribosome-associated mRNAs were immunopurified from specific cell populations of intact seedlings using Arabidopsis thaliana lines expressing a FLAG-epitope tagged ribosomal protein L18 (FLAG-RPL18) via developmentally regulated promoters. The profiling of mRNAs in ribosome complexes, referred to as the translatome, identified differentially expressed mRNAs in 21 cell populations defined by cell-specific expression of FLAG-RPL18. Phloem companion cells of the root and shoot had the most distinctive translatomes. When seedlings were exposed to a brief period of hypoxia, a pronounced reprioritization of mRNA enrichment in the cell-specific translatomes occurred, including a ubiquitous rise in 49 mRNAs encoding transcription factors, signaling proteins, anaerobic metabolism enzymes, and uncharacterized proteins. Translatome profiling also exposed an intricate molecular signature of transcription factor (TF) family member mRNAs that was markedly reconfigured by hypoxia at global and cell-specific levels. In addition to the demonstration of the complexity and plasticity of cell-specific populations of ribosome-associated mRNAs, this study provides an in silico dataset for recognition of differentially expressed genes at the cell-, region-, and organ-specific levels.
format Articulo
Articulo
author Mustroph, Angelika
Zanetti, María Eugenia
Jang, Charles J.H.
Holtan, Hans E.
Repetti, Peter P.
Galbraith, David W.
Girke, Thomas
Bailey-Serres, Julia
author_facet Mustroph, Angelika
Zanetti, María Eugenia
Jang, Charles J.H.
Holtan, Hans E.
Repetti, Peter P.
Galbraith, David W.
Girke, Thomas
Bailey-Serres, Julia
author_sort Mustroph, Angelika
title Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis
title_short Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis
title_full Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis
title_fullStr Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis
title_full_unstemmed Profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in Arabidopsis
title_sort profiling translatomes of discrete cell populations resolves altered cellular priorities during hypoxia in arabidopsis
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82755
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