Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress

Bacteria of the genus Azospirillum are free-living nitrogen-fixing, rhizobacteria that are found in close association with plant roots, where they exert beneficial effects on plant growth and yield in many crops of agronomic importance. Unlike other bacteria, little is known about the genetics and b...

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Autores principales:	Jofre, Edgardo, Fischer, Sonia, Príncipe, Analía, Castro, Marina, Ferrari, Walter, Lagares, Antonio, Mori, Gladys
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2009
Materias:	Ciencias Exactas Azospirillum brasilense Cell division D-alanine-d-alanine ligase Exopolysaccharide Salinity tolerance
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82749
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82749
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Azospirillum brasilense Cell division D-alanine-d-alanine ligase Exopolysaccharide Salinity tolerance
spellingShingle	Ciencias Exactas Azospirillum brasilense Cell division D-alanine-d-alanine ligase Exopolysaccharide Salinity tolerance Jofre, Edgardo Fischer, Sonia Príncipe, Analía Castro, Marina Ferrari, Walter Lagares, Antonio Mori, Gladys Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
topic_facet	Ciencias Exactas Azospirillum brasilense Cell division D-alanine-d-alanine ligase Exopolysaccharide Salinity tolerance
description	Bacteria of the genus Azospirillum are free-living nitrogen-fixing, rhizobacteria that are found in close association with plant roots, where they exert beneficial effects on plant growth and yield in many crops of agronomic importance. Unlike other bacteria, little is known about the genetics and biochemistry of exopolysaccharides in Azospirillum brasilense. In an attempt to characterize genes associated with exopolysaccharides production, we generated an A. brasilense Cd Tn5 mutant that showed exopolysaccharides overproduction, decreased tolerance to saline conditions, altered cell morphology, and increased sensitivity to detergents. Genetic characterization showed that the Tn5 was inserted within a ddlB gene encoding for a d-alanine-d-alanine ligase, and located upstream of the ftsQAZ gene cluster responsible for cell division in different bacteria. Heterologous complementation of the ddlB Tn5 mutant restored the exopolysaccharides production to wild-type levels and the ability to grow in the presence of detergents, but not the morphology and growth characteristics of the wild-type bacteria, suggesting a polar effect of Tn5 on the fts genes. This result and the construction of a nonpolar ddlB mutant provide solid evidence of the presence of transcriptional coupling between a gene associated with peptidoglycan biosynthesis and the fts genes required to control cell division.
format	Articulo Articulo
author	Jofre, Edgardo Fischer, Sonia Príncipe, Analía Castro, Marina Ferrari, Walter Lagares, Antonio Mori, Gladys
author_facet	Jofre, Edgardo Fischer, Sonia Príncipe, Analía Castro, Marina Ferrari, Walter Lagares, Antonio Mori, Gladys
author_sort	Jofre, Edgardo
title	Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
title_short	Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
title_full	Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
title_fullStr	Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
title_full_unstemmed	Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
title_sort	mutation in a d-alanine-d-alanine ligase of azospirillum brasilense cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress
publishDate	2009
url	http://sedici.unlp.edu.ar/handle/10915/82749
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Mutation in a D-alanine-D-alanine ligase of Azospirillum brasilense Cd results in an overproduction of exopolysaccharides and a decreased tolerance to saline stress

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