A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association

Legume plants are able to establish a symbiotic relationship with soil bacteria from the genus Rhizobium, leading to the formation of nitrogen-fixing root nodules. Successful nodulation requires both the formation of infection threads (ITs) in the root epidermis and the activation of cell division i...

Descripción completa

Detalles Bibliográficos
Autores principales: Blanco, Flavio Antonio, Peltzer Meschini, Eitel, Zanetti, María Eugenia, Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Exactas
Rhizobium
Simbiosis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82746
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82746
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Rhizobium
Simbiosis
spellingShingle Ciencias Exactas
Rhizobium
Simbiosis
Blanco, Flavio Antonio
Peltzer Meschini, Eitel
Zanetti, María Eugenia
Aguilar, Orlando Mario
A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
topic_facet Ciencias Exactas
Rhizobium
Simbiosis
description Legume plants are able to establish a symbiotic relationship with soil bacteria from the genus Rhizobium, leading to the formation of nitrogen-fixing root nodules. Successful nodulation requires both the formation of infection threads (ITs) in the root epidermis and the activation of cell division in the cortex to form the nodule primordium. This study describes the characterization of RabA2, a common bean [Phaseolus vulgaris) cDNA previously isolated as differentially expressed in root hairs infected with Rhizobium etli, which encodes a protein highly similar to small GTPases of the RabA2 subfamily. This gene is expressed in roots, particularly in root hairs, where the protein was found to be associated with vesicles that move along the cell. The role of this gene during nodulation has been studied in common bean transgenic roots using a reverse genetic approach. Examination of root morphology in RabA2 RNA interference (RNAi) plants revealed that the number and length of the root hairs were severely reduced in these plants. Upon inoculation with R. etli, nodulation was completely impaired and no induction of early nodulation genes (ENODs), such as ERN1, ENOD40, and Hap5, was detected in silenced hairy roots. Moreover, RabA2 RNAi plants failed to induce root hair deformation and to initiate ITs, indicating that morphological changes that precede bacterial infection are compromised in these plants. We propose that RabA2 acts in polar growth of root hairs and is required for reorientation of the root hair growth axis during bacterial infection.
format Articulo
Articulo
author Blanco, Flavio Antonio
Peltzer Meschini, Eitel
Zanetti, María Eugenia
Aguilar, Orlando Mario
author_facet Blanco, Flavio Antonio
Peltzer Meschini, Eitel
Zanetti, María Eugenia
Aguilar, Orlando Mario
author_sort Blanco, Flavio Antonio
title A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
title_short A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
title_full A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
title_fullStr A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
title_full_unstemmed A small GTPase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
title_sort small gtpase of the rab family is required for root hair formation and preinfection stages of the common bean-rhizobium symbiotic association
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82746
work_keys_str_mv AT blancoflavioantonio asmallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT peltzermeschinieitel asmallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT zanettimariaeugenia asmallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT aguilarorlandomario asmallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT blancoflavioantonio smallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT peltzermeschinieitel smallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT zanettimariaeugenia smallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
AT aguilarorlandomario smallgtpaseoftherabfamilyisrequiredforroothairformationandpreinfectionstagesofthecommonbeanrhizobiumsymbioticassociation
bdutipo_str Repositorios
_version_ 1764820488586002434

Ejemplares similares