Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells

We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Matzkin, María E., Gonzalez-Calvar, Silvia I., Mayerhofer, Artur, Calandra, Ricardo S., Frungieri, Mónica B.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2009
Materias:	Ciencias Médicas prostaglandin-endoperoxide synthase 2 prostaglandins
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82706
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82706
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas prostaglandin-endoperoxide synthase 2 prostaglandins
spellingShingle	Ciencias Médicas prostaglandin-endoperoxide synthase 2 prostaglandins Matzkin, María E. Gonzalez-Calvar, Silvia I. Mayerhofer, Artur Calandra, Ricardo S. Frungieri, Mónica B. Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
topic_facet	Ciencias Médicas prostaglandin-endoperoxide synthase 2 prostaglandins
description	We have previously observed expression of prostaglandin-endoperoxide synthase 2 (PTGS2), the key enzyme in the biosynthesis of prostaglandins (PGs), in reproductively active Syrian hamster Leydig cells, and reported an inhibitory role of PGF 2α on hamster testicular steroidogenesis. In this study, we further investigated PTGS2 expression in hamster Leydig cells during sexual development and photoperiodic gonadal regression. Since PTGS2 is mostly expressed in pubertal and reproductively active adult hamsters with high circulating levels of LH and androgens, we studied the role of these hormones in the regulation/maintenance of testicular PTGS2/PGF 2α. In active hamster Leydig cells, LH/hCG and testosterone induced PTGS2 and PGF 2α production, and their actions were abolished by the antiandrogen bicalutamide (Bi). These results indicate that LH does not exert a direct effect on PG synthesis. Testosterone also stimulated phosphorylation of the mitogen-activated protein kinase isoforms 3/1 (MAPK3/1) within minutes and hours, but the testosterone metabolite dihydrotestosterone had no effect on PTGS2 and MAPK3/1. Because Bi and U0126, an inhibitor of the MAP kinase kinases 1 and 2 (MAP2K1/2), abolished testosterone actions on MAPK3/1 and PTGS2, our studies suggest that testosterone directly induces PTGS2/PGF 2α in hamster Leydig cells via androgen receptors and a non-classical mechanism that involves MAPK3/1 activation. Since PGF 2α inhibits testosterone production, it might imply the existence of a regulatory loop that is setting a brake on steroidogenesis. Thus, the androgen environment might be crucial for the regulation of testicular PG production at least during sexual development and photoperiodic variations in hamsters.
format	Articulo Articulo
author	Matzkin, María E. Gonzalez-Calvar, Silvia I. Mayerhofer, Artur Calandra, Ricardo S. Frungieri, Mónica B.
author_facet	Matzkin, María E. Gonzalez-Calvar, Silvia I. Mayerhofer, Artur Calandra, Ricardo S. Frungieri, Mónica B.
author_sort	Matzkin, María E.
title	Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_short	Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_full	Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_fullStr	Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_full_unstemmed	Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells
title_sort	testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin f 2α production in hamster leydig cells
publishDate	2009
url	http://sedici.unlp.edu.ar/handle/10915/82706
work_keys_str_mv	AT matzkinmariae testosteroneinductionofprostaglandinendoperoxidesynthase2expressionandprostaglandinf2aproductioninhamsterleydigcells AT gonzalezcalvarsilviai testosteroneinductionofprostaglandinendoperoxidesynthase2expressionandprostaglandinf2aproductioninhamsterleydigcells AT mayerhoferartur testosteroneinductionofprostaglandinendoperoxidesynthase2expressionandprostaglandinf2aproductioninhamsterleydigcells AT calandraricardos testosteroneinductionofprostaglandinendoperoxidesynthase2expressionandprostaglandinf2aproductioninhamsterleydigcells AT frungierimonicab testosteroneinductionofprostaglandinendoperoxidesynthase2expressionandprostaglandinf2aproductioninhamsterleydigcells
bdutipo_str	Repositorios
_version_	1764820488546156548

Testosterone induction of prostaglandin-endoperoxide synthase 2 expression and prostaglandin F 2α production in hamster Leydig cells

Ejemplares similares