Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer

The aim of this study was to determine the existence of association between the genetic polymorphisms of metabolizing genes GSTM-1, GSTT-1,and NAT-2,and the presence of mitochondrial genome instability (mtGI) in breast cancer cases. Ninety-four pairs of tumoral/nontumoral breast cancer samples were...

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Detalles Bibliográficos
Autores principales: Pavicic, Walter Hernán, Laguens, Martín Ignacio, Richard, Silvina Mariel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Médicas
genes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82685
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82685
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
genes
spellingShingle Ciencias Médicas
genes
Pavicic, Walter Hernán
Laguens, Martín Ignacio
Richard, Silvina Mariel
Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
topic_facet Ciencias Médicas
genes
description The aim of this study was to determine the existence of association between the genetic polymorphisms of metabolizing genes GSTM-1, GSTT-1,and NAT-2,and the presence of mitochondrial genome instability (mtGI) in breast cancer cases. Ninety-four pairs of tumoral/nontumoral breast cancer samples were analyzed. Our samples showed 40.42% of mtGI by analysis of two D-loop region markers, a (CA)n mtMS starting at the 514-bp position, and four informative MnlI sites between the 16, 108-16, 420-bp. GSTM-1null genotype has shown a significant association with mtGI presence (χ<SUP>2</SUP> = 7.62; P= 0.006) in breast cancer cases; moreover, these genotypes also are related to an increased risk for mtDNA damage (odds ratio (OR) = 3.71 (1.41-9.88); 95% Cornfield confidence interval (CI)). These results suggest that the absence of GSTM-1enzymatic activity favors chemical actions in damaging the mtDNA. Analysis of GSTT-1and NAT-2polymorphisms showed no association with mtGI (χ<SUP>2</SUP> = 0.03; P = 0.87 and χ<SUP>2</SUP> = 2.76; P = 0.09, respectively). The analysis of invasive breast cancer cases showed mtGI in 74.36% of ILC cases (29 of 39 samples), and in only 18.75% (9 out of 48) IDC cases; this result suggests a possible relation between mtDNA mutations and variations in molecular pathways of tumor development.
format Articulo
Articulo
author Pavicic, Walter Hernán
Laguens, Martín Ignacio
Richard, Silvina Mariel
author_facet Pavicic, Walter Hernán
Laguens, Martín Ignacio
Richard, Silvina Mariel
author_sort Pavicic, Walter Hernán
title Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
title_short Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
title_full Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
title_fullStr Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
title_full_unstemmed Analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
title_sort analysis association between mitochondrial genome instability and xenobiotic metabolizing genes in human breast cancer
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82685
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AT laguensmartinignacio analysisassociationbetweenmitochondrialgenomeinstabilityandxenobioticmetabolizinggenesinhumanbreastcancer
AT richardsilvinamariel analysisassociationbetweenmitochondrialgenomeinstabilityandxenobioticmetabolizinggenesinhumanbreastcancer
bdutipo_str Repositorios
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