Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis

Bordetella pertussis is a re-emerging human respiratory pathogen whose infectious process is not fully understood, hampering the design of effective vaccines. The nature of bacterial attachment to host cells is a key event in the outcome of the infection. However, host cell receptors involved in B....

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Detalles Bibliográficos
Autores principales: Lamberti, Yanina Andrea, Álvarez Hayes, Jimena, Pérez Vidakovics, María Laura Anabella, Rodríguez, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Bioquímica
Attachment
Bordetella pertussis
Cholesterol
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82684
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82684
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Attachment
Bordetella pertussis
Cholesterol
spellingShingle Bioquímica
Attachment
Bordetella pertussis
Cholesterol
Lamberti, Yanina Andrea
Álvarez Hayes, Jimena
Pérez Vidakovics, María Laura Anabella
Rodríguez, María Eugenia
Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis
topic_facet Bioquímica
Attachment
Bordetella pertussis
Cholesterol
description Bordetella pertussis is a re-emerging human respiratory pathogen whose infectious process is not fully understood, hampering the design of effective vaccines. The nature of bacterial attachment to host cells is a key event in the outcome of the infection. However, host cell receptors involved in B. pertussis colonization of the respiratory tract are still under investigation. Here, we report that cholesterol-rich domains are involved in B. pertussis adhesion to epithelial cells. Treatment of A549 cells with cholesterol-sequestering drugs such as methyl-β-cyclodextrin, nystatin, or filipin resulted in a significant decrease of B. pertussis attachment. Confocal laser microscopy studies showed B. pertussis associated with cholesterol-rich domains. Accordingly, B. pertussis was found in detergent-resistant membrane domain fractions isolated from bacterial-infected A549 cells. Our results indicate a main role of filamentous hemagglutinin, an environmentally regulated virulence factor, in this interaction, and a specific affinity for cholesterol, one of the major components of traqueal secretions, which might additionally contribute to the effective colonization of the respiratory tract.
format Articulo
Articulo
author Lamberti, Yanina Andrea
Álvarez Hayes, Jimena
Pérez Vidakovics, María Laura Anabella
Rodríguez, María Eugenia
author_facet Lamberti, Yanina Andrea
Álvarez Hayes, Jimena
Pérez Vidakovics, María Laura Anabella
Rodríguez, María Eugenia
author_sort Lamberti, Yanina Andrea
title Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis
title_short Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis
title_full Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis
title_fullStr Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis
title_full_unstemmed Cholesterol-dependent attachment of human respiratory cells by Bordetella pertussis
title_sort cholesterol-dependent attachment of human respiratory cells by bordetella pertussis
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82684
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AT alvarezhayesjimena cholesteroldependentattachmentofhumanrespiratorycellsbybordetellapertussis
AT perezvidakovicsmarialauraanabella cholesteroldependentattachmentofhumanrespiratorycellsbybordetellapertussis
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