Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses

Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucl...

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Autores principales: Nakauchi, Mina, Fukushi, Shuetsu, Saijo, Masayuki, Mizutani, Tetsuya, Ure, Agustín Enrique, Romanowski, Víctor, Kurane, Ichiro, Morikawa, Shigeru
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Biología
Arenavirus del Nuevo Mundo
monoclonal antibodies
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82675
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82675
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Arenavirus del Nuevo Mundo
monoclonal antibodies
spellingShingle Biología
Arenavirus del Nuevo Mundo
monoclonal antibodies
Nakauchi, Mina
Fukushi, Shuetsu
Saijo, Masayuki
Mizutani, Tetsuya
Ure, Agustín Enrique
Romanowski, Víctor
Kurane, Ichiro
Morikawa, Shigeru
Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
topic_facet Biología
Arenavirus del Nuevo Mundo
monoclonal antibodies
description Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus.
format Articulo
Articulo
author Nakauchi, Mina
Fukushi, Shuetsu
Saijo, Masayuki
Mizutani, Tetsuya
Ure, Agustín Enrique
Romanowski, Víctor
Kurane, Ichiro
Morikawa, Shigeru
author_facet Nakauchi, Mina
Fukushi, Shuetsu
Saijo, Masayuki
Mizutani, Tetsuya
Ure, Agustín Enrique
Romanowski, Víctor
Kurane, Ichiro
Morikawa, Shigeru
author_sort Nakauchi, Mina
title Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
title_short Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
title_full Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
title_fullStr Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
title_full_unstemmed Characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses
title_sort characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by south american arenaviruses
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82675
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