A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis

Legume plants are able to interact symbiotically with soil bacteria to form nitrogen-fixing root nodules. Although specific recognition between rhizobia and legume species has been extensively characterized, plant molecular determinants that govern the preferential colonization by different strains...

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Autores principales: Zanetti, María Eugenia, Blanco, Flavio Antonio, Beker, María Pía, Battaglia, Marina, Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Exactas
Rhizobium etli
RNA interference
CCAAT-Binding Factor
Cell Nucleus
Gene Expression Profiling
Phaseolus
Plant Roots
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82604
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82604
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Rhizobium etli
RNA interference
CCAAT-Binding Factor
Cell Nucleus
Gene Expression Profiling
Phaseolus
Plant Roots
spellingShingle Ciencias Exactas
Rhizobium etli
RNA interference
CCAAT-Binding Factor
Cell Nucleus
Gene Expression Profiling
Phaseolus
Plant Roots
Zanetti, María Eugenia
Blanco, Flavio Antonio
Beker, María Pía
Battaglia, Marina
Aguilar, Orlando Mario
A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis
topic_facet Ciencias Exactas
Rhizobium etli
RNA interference
CCAAT-Binding Factor
Cell Nucleus
Gene Expression Profiling
Phaseolus
Plant Roots
description Legume plants are able to interact symbiotically with soil bacteria to form nitrogen-fixing root nodules. Although specific recognition between rhizobia and legume species has been extensively characterized, plant molecular determinants that govern the preferential colonization by different strains within a single rhizobium species have received little attention. We found that the C subunit of the heterotrimeric nuclear factor NF-Y from common bean (Phaseolus vulgaris) NF-YC1 plays a key role in the improved nodulation seen by more efficient strains of rhizobia. Reduction of NF-YC1 transcript levels by RNA interference (RNAi) in Agrobacterium rhizogenes-induced hairy roots leads to the arrest of nodule development and defects in the infection process with either high or low efficiency strains. Induction of three G2/M transition cell cycle genes in response to rhizobia was impaired or attenuated in NF-YC1 RNAi roots, suggesting that this transcription factor might promote nodule development by activating cortical cell divisions. Furthermore, overexpression of this gene has a positive impact on nodulation efficiency and selection of Rhizobium etli strains that are naturally less efficient and bad competitors. Our findings suggest that this transcription factor might be part of a mechanism that links nodule organogenesis with an early molecular dialogue that selectively discriminates between high- and low-quality symbiotic partners, which holds important implications for optimizing legume performance.
format Articulo
Articulo
author Zanetti, María Eugenia
Blanco, Flavio Antonio
Beker, María Pía
Battaglia, Marina
Aguilar, Orlando Mario
author_facet Zanetti, María Eugenia
Blanco, Flavio Antonio
Beker, María Pía
Battaglia, Marina
Aguilar, Orlando Mario
author_sort Zanetti, María Eugenia
title A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis
title_short A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis
title_full A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis
title_fullStr A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis
title_full_unstemmed A C subunit of the plant nuclear factor NF-Y required for rhizobial infection and nodule development affects partner selection in the common bean-Rhizobium etli Symbiosis
title_sort c subunit of the plant nuclear factor nf-y required for rhizobial infection and nodule development affects partner selection in the common bean-rhizobium etli symbiosis
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/82604
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