Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus

Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined i...

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Detalles Bibliográficos
Autores principales:	Velázquez, K., Renovell, A., Comellas, M., Serra, P., García, María Laura, Pina, J. A., Navarro, L., Moreno, P., Guerri, J.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Biología Bark scaling disease of citrus Citrus sinensis SiRNA Viral symptomatology Virus titre
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82549
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82549
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Bark scaling disease of citrus Citrus sinensis SiRNA Viral symptomatology Virus titre
spellingShingle	Biología Bark scaling disease of citrus Citrus sinensis SiRNA Viral symptomatology Virus titre Velázquez, K. Renovell, A. Comellas, M. Serra, P. García, María Laura Pina, J. A. Navarro, L. Moreno, P. Guerri, J. Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus
topic_facet	Biología Bark scaling disease of citrus Citrus sinensis SiRNA Viral symptomatology Virus titre
description	Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26/18°C or 32/26°C (day/night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32/26°C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26/18°C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32/26°C, with the ratio of CPsV-sRNA/vRNA being higher at 32/26°C than at 26/18°C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.
format	Articulo Articulo
author	Velázquez, K. Renovell, A. Comellas, M. Serra, P. García, María Laura Pina, J. A. Navarro, L. Moreno, P. Guerri, J.
author_facet	Velázquez, K. Renovell, A. Comellas, M. Serra, P. García, María Laura Pina, J. A. Navarro, L. Moreno, P. Guerri, J.
author_sort	Velázquez, K.
title	Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus
title_short	Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus
title_full	Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus
title_fullStr	Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus
title_full_unstemmed	Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus
title_sort	effect of temperature on rna silencing of a negative-stranded rna plant virus: citrus psorosis virus
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/82549
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Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus

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