Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines

<i>Solanum bonariense</i> intoxication is characterized by cerebellar neuronal vacuolation, degeneration, and necrosis. Cerebellar Purkinje cells seem especially susceptible, but more research is needed to determine the pathogenesis of neuronal necrosis and the mechanism of Purkinje cell...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Verdes, J. M., Moraña, J. A., Battes, D., Gutiérrez, F., Guerrero, F., Goicoa, A., Fidalgo, L. E., Barbeito, Claudio Gustavo, Zanuzzi, Carolina Natalia, Portiansky, Enrique Leo, Gimeno, Eduardo Juan
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Veterinarias
Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
Excitotoxicity
Plant poisoning
Purkinje cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82543
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82543
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
Excitotoxicity
Plant poisoning
Purkinje cells
spellingShingle Ciencias Veterinarias
Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
Excitotoxicity
Plant poisoning
Purkinje cells
Verdes, J. M.
Moraña, J. A.
Battes, D.
Gutiérrez, F.
Guerrero, F.
Goicoa, A.
Fidalgo, L. E.
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
topic_facet Ciencias Veterinarias
Apoptosis
Calbindin D28k
Cattle
Cerebellar degeneration
Excitotoxicity
Plant poisoning
Purkinje cells
description <i>Solanum bonariense</i> intoxication is characterized by cerebellar neuronal vacuolation, degeneration, and necrosis. Cerebellar Purkinje cells seem especially susceptible, but more research is needed to determine the pathogenesis of neuronal necrosis and the mechanism of Purkinje cell susceptibility. Calbindin D28k (CbD28k) is highly expressed in Purkinje cells and has been used as a marker for normal and degenerative Purkinje cells. The goal of this study was to describe <i>S bonariense</i>-induced disease by ascertaining Purkinje cell-specific degenerative changes using CbD28k expression and to correlate this with apoptosis in Purkinje cells, as determined using TUNEL (transferase-mediated dUTP-biotin nick end-labeling) and ultrastructural changes. In all cases, an increase in both dose and duration of <i>S bonariense</i> intoxication resulted in a decrease in the number of Purkinje cells. CbD28k immunohistochemistry was an excellent marker for Purkinje cells because immunoreactivity did not change in normal or degenerative tissues. This finding suggests that excessive calcium excitatory stimulation does not induce rapid neuronal degeneration and death. As found in previous studies, TUNEL tests and electron microscopy suggest that Purkinje cell degeneration and death are not occurring via an apoptotic process. These findings suggest that <i>S bonariense</i> poisoning induces progressive Purkinje cell death that is not mediated by excitotoxicity or apoptotic activation.
format Articulo
Articulo
author Verdes, J. M.
Moraña, J. A.
Battes, D.
Gutiérrez, F.
Guerrero, F.
Goicoa, A.
Fidalgo, L. E.
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_facet Verdes, J. M.
Moraña, J. A.
Battes, D.
Gutiérrez, F.
Guerrero, F.
Goicoa, A.
Fidalgo, L. E.
Barbeito, Claudio Gustavo
Zanuzzi, Carolina Natalia
Portiansky, Enrique Leo
Gimeno, Eduardo Juan
author_sort Verdes, J. M.
title Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
title_short Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
title_full Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
title_fullStr Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
title_full_unstemmed Calbindin D28k Expression and the Absence of Apoptosis in the Cerebellum of Solatium bonariense L-lntoxicated Bovines
title_sort calbindin d28k expression and the absence of apoptosis in the cerebellum of solatium bonariense l-lntoxicated bovines
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/82543
work_keys_str_mv AT verdesjm calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT moranaja calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT battesd calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT gutierrezf calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT guerrerof calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT goicoaa calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT fidalgole calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT barbeitoclaudiogustavo calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT zanuzzicarolinanatalia calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT portianskyenriqueleo calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
AT gimenoeduardojuan calbindind28kexpressionandtheabsenceofapoptosisinthecerebellumofsolatiumbonariensellntoxicatedbovines
bdutipo_str Repositorios
_version_ 1764820488386772994

Ejemplares similares