Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology

Summary During intestinal transplant (ITx) operation, intestinal lymphatics are not reconstituted. Consequently, trafficking immune cells drain freely into the abdominal cavity. Our aim was to evaluate whether leucocytes migrating from a transplanted intestine could be recovered from the abdominal d...

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Detalles Bibliográficos
Autores principales: Meier, Dominik, Cagnolati, Hernán, Ramisch, Diego, Rumbo, Carolina, Chirdo, Fernando Gabriel, Docena, Guillermo Horacio, Gondolesi, Gabriel, Rumbo, Martín
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Médicas
abdominal cavity drainage
immune cells
intestinal transplant
lymphocytes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82525
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82525
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
abdominal cavity drainage
immune cells
intestinal transplant
lymphocytes
spellingShingle Ciencias Médicas
abdominal cavity drainage
immune cells
intestinal transplant
lymphocytes
Meier, Dominik
Cagnolati, Hernán
Ramisch, Diego
Rumbo, Carolina
Chirdo, Fernando Gabriel
Docena, Guillermo Horacio
Gondolesi, Gabriel
Rumbo, Martín
Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
topic_facet Ciencias Médicas
abdominal cavity drainage
immune cells
intestinal transplant
lymphocytes
description Summary During intestinal transplant (ITx) operation, intestinal lymphatics are not reconstituted. Consequently, trafficking immune cells drain freely into the abdominal cavity. Our aim was to evaluate whether leucocytes migrating from a transplanted intestine could be recovered from the abdominal draining fluid collected by a peritoneal drainage system in the early post-ITx period, and to determine potential applications of the assessment of draining cellular populations. The cell composition of the abdominal draining fluid was analysed during the first 11 post-ITx days. Using flow cytometry, immune cells from blood and draining fluid samples obtained the same day showed an almost complete lymphopenia in peripheral blood, whereas CD3+CD4+CD8 -, CD3+CD4-CD8+ and human leucocyte antigen D-related (HLA-DR)+CD19+ lymphocytes were the main populations in the draining fluid. Non-complicated recipients evolved from a mixed leucocyte pattern including granulocytes, monocytes and lymphocytes to an exclusively lymphocytic pattern along the first post-ITx week. At days 1-2 post-Itx, analysis by short tandem repeats fingerprinting of CD3 +CD8+ sorted T cells from draining fluid indicated that 50% of cells were from graft origin, whereas by day 11 post-ITx this proportion decreased to fewer than 1%. Our results show for the first time that the abdominal drainage fluid contains mainly immune cells trafficking from the implanted intestine, providing the opportunity to sample lymphocytes draining from the grafted organ along the post-ITx period. Therefore, this analysis may provide information useful for understanding ITx immunobiology and eventually could also be of interest for clinical management.
format Articulo
Articulo
author Meier, Dominik
Cagnolati, Hernán
Ramisch, Diego
Rumbo, Carolina
Chirdo, Fernando Gabriel
Docena, Guillermo Horacio
Gondolesi, Gabriel
Rumbo, Martín
author_facet Meier, Dominik
Cagnolati, Hernán
Ramisch, Diego
Rumbo, Carolina
Chirdo, Fernando Gabriel
Docena, Guillermo Horacio
Gondolesi, Gabriel
Rumbo, Martín
author_sort Meier, Dominik
title Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
title_short Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
title_full Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
title_fullStr Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
title_full_unstemmed Analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
title_sort analysis of immune cells draining from the abdominal cavity as a novel tool to study intestinal transplant immunobiology
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/82525
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bdutipo_str Repositorios
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