Immunohistochemical evidence of Muc1 expression during rat embryonic development

During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat developmen...

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Autores principales:	Lacunza, Ezequiel, Ferretti, Valeria Alejandra, Barbeito, Claudio Gustavo, Segal-Eiras, Amada, Croce, María Virginia
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82440
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82440
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat
spellingShingle	Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat Lacunza, Ezequiel Ferretti, Valeria Alejandra Barbeito, Claudio Gustavo Segal-Eiras, Amada Croce, María Virginia Immunohistochemical evidence of Muc1 expression during rat embryonic development
topic_facet	Ciencias Veterinarias Ciencias Médicas Embryonic development Immunohistochemistry Muc1/MUC1 Rat
description	During embryonic development, studies on mouse and human embryos have established that Muc1/MUC1 expression coincides with the onset of epithelial sheet and glandular formation. This study aimed therefore at evaluating the temporal and spatial expression of Muc1 at different stages of rat development. In this report, 80 animals were included: 64 rat foetuses at 13, 14, 15, 16, 17, 18, 19 and 20 days of gestation from pregnant females (WKAH/Hok), 8 embryos each stage. Standard immunohistochemistry was performed using anti-MUC1 cytoplasmic tail polyclonal antibody (CT33). The reaction was considered positive when more than 5% of the cells were stained; reaction patterns were: L = linear, membrane, C = cytoplasmic and M = mixed; nuclear staining was also recorded. Intensity was graded as negative (-), low (+), moderate (++) and strong (+++). Muc1 expression was observed with a low intensity on 13th day (13 D) in the stomach, lung and kidney; at 14 d, small intestine and pancreas were also reactive; at 16 D, liver and esophagus and at 18 D, trachea and salivary glands. During the development, intensity increased while the pattern of expression changed: at the first days of gestation, it was predominantly linear and apical while during further development an increase in cytoplasmic expression was observed. Trachea, stomach, kidney and lung epithelia were the more reactive tissues. In specimens belonging to neonates and adults, all tissues analyzed showed similar Muc1 expression. The findings of this study assess that Muc1 is highly expressed in the epithelial rat embryonic development.
format	Articulo Articulo
author	Lacunza, Ezequiel Ferretti, Valeria Alejandra Barbeito, Claudio Gustavo Segal-Eiras, Amada Croce, María Virginia
author_facet	Lacunza, Ezequiel Ferretti, Valeria Alejandra Barbeito, Claudio Gustavo Segal-Eiras, Amada Croce, María Virginia
author_sort	Lacunza, Ezequiel
title	Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_short	Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_full	Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_fullStr	Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_full_unstemmed	Immunohistochemical evidence of Muc1 expression during rat embryonic development
title_sort	immunohistochemical evidence of muc1 expression during rat embryonic development
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/82440
work_keys_str_mv	AT lacunzaezequiel immunohistochemicalevidenceofmuc1expressionduringratembryonicdevelopment AT ferrettivaleriaalejandra immunohistochemicalevidenceofmuc1expressionduringratembryonicdevelopment AT barbeitoclaudiogustavo immunohistochemicalevidenceofmuc1expressionduringratembryonicdevelopment AT segaleirasamada immunohistochemicalevidenceofmuc1expressionduringratembryonicdevelopment AT crocemariavirginia immunohistochemicalevidenceofmuc1expressionduringratembryonicdevelopment
bdutipo_str	Repositorios
_version_	1764820488282963968

Immunohistochemical evidence of Muc1 expression during rat embryonic development

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