Effects of photooxidation on membrane integrity in Salix nigra seeds

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Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. T...

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Autores principales:	Roqueiro, Gonzalo, Facorro, Graciela B., Huarte, Mónica G., Rubín de Celis, Emilio, García, Carlos Fernando, Maldonado, Sara, Maroder, Horacio
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Ciencias Naturales Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82435
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82435
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds
spellingShingle	Ciencias Naturales Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds Roqueiro, Gonzalo Facorro, Graciela B. Huarte, Mónica G. Rubín de Celis, Emilio García, Carlos Fernando Maldonado, Sara Maroder, Horacio Effects of photooxidation on membrane integrity in Salix nigra seeds
topic_facet	Ciencias Naturales Embryo membrane integrity Free radicals Orthodox seed Photooxidation Salicaceae seeds Salix nigra Seed chlorophyll Seed lipid peroxidation Thylakoids Willow seeds
description	Background and Aims: Salix nigra seeds are desiccation-tolerant, as are orthodox seeds, although in contrast to other orthodox seeds they lose viability in a few weeks at room temperature. They also differ in that the chloroplasts of the embryo tissues conserve their chlorophyll and endomembranes. The aim of this paper was to investigate the role of chlorophyll in seed deterioration. Methods: Seeds were aged at different light intensities and atmospheric conditions. Mean germination time and normal and total germination were evaluated. The formation of free radicals was assessed using electronic spin resonance spectroscopy, and changes in the fatty acid composition from phospholipids, galactolipids and triglycerides using gas-liquid chromatography. Membrane integrity was studied with electronic spin resonance spin probe techniques, electrolyte leakage and transmission electron microscopy. Key Results: Light and oxygen played an important role in free-radical generation, causing a decrease in normal germination and an increase in mean germination time. Both indices were associated with a decrease in polyunsaturated fatty acids derived from membrane lipids as phospholipids and galactolipids. The detection of damage in thylakoid membranes and an increase in plasmalemma permeability were consistent with the decrease in both types of lipids. Triglycerides remained unchanged. Light-induced damage began in outermost tissues and spread inwards, decreasing normal germination. Conclusions: Salix nigra seeds were very susceptible to photooxidation. The thylakoid membranes appeared to be the first target of the photooxidative process since there were large decreases in galactolipids and both these lipids and the activated chlorophyll are contiguous in the structure of that membrane. Changes in normal germination and mean germination time could be explained by the deteriorative effects of oxidation.
format	Articulo Articulo
author	Roqueiro, Gonzalo Facorro, Graciela B. Huarte, Mónica G. Rubín de Celis, Emilio García, Carlos Fernando Maldonado, Sara Maroder, Horacio
author_facet	Roqueiro, Gonzalo Facorro, Graciela B. Huarte, Mónica G. Rubín de Celis, Emilio García, Carlos Fernando Maldonado, Sara Maroder, Horacio
author_sort	Roqueiro, Gonzalo
title	Effects of photooxidation on membrane integrity in Salix nigra seeds
title_short	Effects of photooxidation on membrane integrity in Salix nigra seeds
title_full	Effects of photooxidation on membrane integrity in Salix nigra seeds
title_fullStr	Effects of photooxidation on membrane integrity in Salix nigra seeds
title_full_unstemmed	Effects of photooxidation on membrane integrity in Salix nigra seeds
title_sort	effects of photooxidation on membrane integrity in salix nigra seeds
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/82435
work_keys_str_mv	AT roqueirogonzalo effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT facorrogracielab effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT huartemonicag effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT rubindecelisemilio effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT garciacarlosfernando effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT maldonadosara effectsofphotooxidationonmembraneintegrityinsalixnigraseeds AT maroderhoracio effectsofphotooxidationonmembraneintegrityinsalixnigraseeds
bdutipo_str	Repositorios
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Effects of photooxidation on membrane integrity in Salix nigra seeds

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