Analytical method validation for in vitro permeation of Calendula officinalis L. gel

The objective of this study was to evaluate preliminary stability, as well as to validate an analytical method for in vitro skin permeation study of a gel containing tincture of Calendula officinalis L., aiming at the implementation and consolidation of methodologies to be used as prototype for futu...

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Autores principales:	Nunes, Kariane M., Bandeira, Elanne S., Ozela, Eliana F., Barbosa, Wagner L.R., Costa, Roseane M.R., Silva Júnior, José O. C.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia analytical method validation; calendula officinalis L.; in vitro cutaneous permeation; rutin Medicina de Hierbas Permeabilidad Geles Fitoterapia
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8238 http://www.latamjpharm.org/resumenes/30/5/LAJOP_30_5_1_14.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8238
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia analytical method validation; calendula officinalis L.; in vitro cutaneous permeation; rutin Medicina de Hierbas Permeabilidad Geles Fitoterapia
spellingShingle	Farmacia analytical method validation; calendula officinalis L.; in vitro cutaneous permeation; rutin Medicina de Hierbas Permeabilidad Geles Fitoterapia Nunes, Kariane M. Bandeira, Elanne S. Ozela, Eliana F. Barbosa, Wagner L.R. Costa, Roseane M.R. Silva Júnior, José O. C. Analytical method validation for in vitro permeation of Calendula officinalis L. gel
topic_facet	Farmacia analytical method validation; calendula officinalis L.; in vitro cutaneous permeation; rutin Medicina de Hierbas Permeabilidad Geles Fitoterapia
description	The objective of this study was to evaluate preliminary stability, as well as to validate an analytical method for in vitro skin permeation study of a gel containing tincture of Calendula officinalis L., aiming at the implementation and consolidation of methodologies to be used as prototype for future development of herbal medicine in the Brazilian Amazon region. The gel showed a satisfactory result during the assessment of its preliminary stability considering that the formulation remained stable throughout cycles at temperatures of 45 °C and 25 °C. Parameters obtained in the validation of the analytical method presented satisfactory results for specificity, linearity (r > 0.99), precision (intra-day and inter-day) with relative standard deviation values (R.S.D. %) below the required maximum of 5 %. Accuracy and robustness were consistent with both the objectives of the work and recommendations of the existing laws, providing the reliability of the data obtained during the skin permeation study, evidenced by the gel tendency to promote the permeation of flavonoids, expressed in rutin, through models of synthetic and biological membrane. However, it was observed that the gel had a higher permeability ratio through synthetic membrane, due to greater cumulative mass of rutin quantified after permeation.
format	Articulo Articulo
author	Nunes, Kariane M. Bandeira, Elanne S. Ozela, Eliana F. Barbosa, Wagner L.R. Costa, Roseane M.R. Silva Júnior, José O. C.
author_facet	Nunes, Kariane M. Bandeira, Elanne S. Ozela, Eliana F. Barbosa, Wagner L.R. Costa, Roseane M.R. Silva Júnior, José O. C.
author_sort	Nunes, Kariane M.
title	Analytical method validation for in vitro permeation of Calendula officinalis L. gel
title_short	Analytical method validation for in vitro permeation of Calendula officinalis L. gel
title_full	Analytical method validation for in vitro permeation of Calendula officinalis L. gel
title_fullStr	Analytical method validation for in vitro permeation of Calendula officinalis L. gel
title_full_unstemmed	Analytical method validation for in vitro permeation of Calendula officinalis L. gel
title_sort	analytical method validation for in vitro permeation of calendula officinalis l. gel
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8238 http://www.latamjpharm.org/resumenes/30/5/LAJOP_30_5_1_14.pdf
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Analytical method validation for in vitro permeation of Calendula officinalis L. gel

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