Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo
A simple and specific method was validated for quantification of boldine in fluid extract of boldo (Peumus boldus Mol.) using high-performance liquid chromatography. A reversed-phase C<sub>18</sub> , Phenomenex® (150 x 4.6 mm, 4 µm) column was employed. The mobile phase consisted of 0....
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8224 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_2_11.pdf |
| Aporte de: |
| id |
I19-R120-10915-8224 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Extractos Vegetales boldine; high-performance liquid chromatography; Peumus boldus Mol.; quality control; validation Cromatografía Control de Calidad |
| spellingShingle |
Farmacia Extractos Vegetales boldine; high-performance liquid chromatography; Peumus boldus Mol.; quality control; validation Cromatografía Control de Calidad Casal, Francieli Mallmann, Michele Pedroni, Helen C. Grazziotin, Manoela M. Tasso, Leandro Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| topic_facet |
Farmacia Extractos Vegetales boldine; high-performance liquid chromatography; Peumus boldus Mol.; quality control; validation Cromatografía Control de Calidad |
| description |
A simple and specific method was validated for quantification of boldine in fluid extract of
boldo (Peumus boldus Mol.) using high-performance liquid chromatography. A reversed-phase C<sub>18</sub>
, Phenomenex® (150 x 4.6 mm, 4 µm) column was employed. The mobile phase consisted of 0.1 % trifluoroacetic acid and acetonitrile (78:22, v/v) at a flow rate of 0.8 mL/min. The column was maintained at 30
°C and the boldine peak detection was performed at a wavelength of 281 nm. The parameters used in the
validation process were: linearity, specificity, precision, accuracy, limit of detection, limit of quantification
and robustness. The validated method was selective and linear (r≥0.9991) for boldine concentration considering 5.0, 10.0, 15.0, 20.0 and 25.0 micro;g/mL. The recovery ranged from 90.93 % to 96.24 % and the limit
of quantification was 2.41 micro;g/mL. The precision determined was reported as RSD (1.73 %). The method
can be successfully applied to measure boldine concentrations in Boldo extract and be included in routine
analysis of quality control. |
| format |
Articulo Comunicacion |
| author |
Casal, Francieli Mallmann, Michele Pedroni, Helen C. Grazziotin, Manoela M. Tasso, Leandro |
| author_facet |
Casal, Francieli Mallmann, Michele Pedroni, Helen C. Grazziotin, Manoela M. Tasso, Leandro |
| author_sort |
Casal, Francieli |
| title |
Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| title_short |
Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| title_full |
Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| title_fullStr |
Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| title_full_unstemmed |
Validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| title_sort |
validation of a high performance liquid chromatographic method for quantitative determination of boldine in fluid extract of boldo |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8224 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_2_11.pdf |
| work_keys_str_mv |
AT casalfrancieli validationofahighperformanceliquidchromatographicmethodforquantitativedeterminationofboldineinfluidextractofboldo AT mallmannmichele validationofahighperformanceliquidchromatographicmethodforquantitativedeterminationofboldineinfluidextractofboldo AT pedronihelenc validationofahighperformanceliquidchromatographicmethodforquantitativedeterminationofboldineinfluidextractofboldo AT grazziotinmanoelam validationofahighperformanceliquidchromatographicmethodforquantitativedeterminationofboldineinfluidextractofboldo AT tassoleandro validationofahighperformanceliquidchromatographicmethodforquantitativedeterminationofboldineinfluidextractofboldo |
| bdutipo_str |
Repositorios |
| _version_ |
1764820488039694338 |