Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)

Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi a...

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Detalles Bibliográficos
Autores principales:	Allegrucci, Natalia, Velázquez, María Silvana, Russo, María Leticia, Pérez, Emilia, Scorsetti, Ana Clara
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Botánica Beauveria bassiana Biocontrol Endophytes Fungal entomopathogens Solanum lycopersicum Tuta absoluta
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82229
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82229
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Botánica Beauveria bassiana Biocontrol Endophytes Fungal entomopathogens Solanum lycopersicum Tuta absoluta
spellingShingle	Botánica Beauveria bassiana Biocontrol Endophytes Fungal entomopathogens Solanum lycopersicum Tuta absoluta Allegrucci, Natalia Velázquez, María Silvana Russo, María Leticia Pérez, Emilia Scorsetti, Ana Clara Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
topic_facet	Botánica Beauveria bassiana Biocontrol Endophytes Fungal entomopathogens Solanum lycopersicum Tuta absoluta
description	Fungal entomopathogens can naturally regulate populations of various insects. The entomopathogen Beauveria bassiana (Bals.- Criv.) Vuill. is also able to endophytically colonize different plants. Endophytic colonization by entomopathogens may provide a source of indirect interactions between fungi and insects and has been associated with the ability of the fungus to control insect pests. The tomato leaf miner, Tuta absoluta (Meyrick) is considered one of the most devastating pests of tomato (Solanum lycopersicum L.) and its difficult control is due to its miner habit, short life cycle, and high reproductive capacity. The aims of this study were: (i) to use three different techniques, i.e. leaf spraying, seed immersion and root dipping, for the endophytic inoculation of B. bassiana in tomato plants; and (ii) to assess the effect of B. bassiana on tomato leaf consumption and mortality of T. absoluta after inoculation. The percentage of colonization by B. bassiana was assessed 7, 14 and 28 days after inoculation. All inoculation techniques employed allowed the recovery of B. bassiana, although our results showed significant differences between techniques. Leaf spraying was the most effective, with the highest percentage of colonization recorded 7 days after inoculation. We also evaluated (i) the effect on the mortality of T. absoluta by direct contact with conidia of B. bassiana, and (ii) the effect on tomato leaf consumption and mortality by indirect contact through ingestion of inoculated plant tissues with B. bassiana. Mortality bioassays showed that B. bassiana infected T. absoluta, either by direct contact or indirectly, via ingestion of inoculated tomato leaves. Direct contact showed a higher percentage of mortality and a lower median survival time (MST) than indirect contact. Significant differences in the mortality percentages of T. absoluta after exposure with B. bassiana were found among the treatments and the control. Our results suggest that the endophytic inoculation of B. bassiana in tomato crops provides the basis for further investigation, which should focus on the virulence of the endophytic B. bassiana against T. absoluta.
format	Articulo Articulo
author	Allegrucci, Natalia Velázquez, María Silvana Russo, María Leticia Pérez, Emilia Scorsetti, Ana Clara
author_facet	Allegrucci, Natalia Velázquez, María Silvana Russo, María Leticia Pérez, Emilia Scorsetti, Ana Clara
author_sort	Allegrucci, Natalia
title	Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_short	Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_full	Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_fullStr	Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_full_unstemmed	Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)
title_sort	endophytic colonisation of tomato by the entomopathogenic fungus beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer tuta absoluta (lepidoptera: gelechiidae)
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/82229
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Endophytic colonisation of tomato by the entomopathogenic fungus Beauveria bassiana: the use of different inoculation techniques and their effects on the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae)

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