Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma

A sensitive and specific high performance reversed phase liquid chromatographic method was developed for quantification of citicoline monosodium (CTM) in human plasma. The active drug was isocratically eluted at a flow rate of 1 ml/min at ambient temperature in a nucleosil C18 analytical column with...

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Detalles Bibliográficos
Autores principales:	Bindaiya, Shailendra K., Sahu, Kapendra, Bhaisare, Mukesh, Karthikeyan, Chandrabose, Moorthy, N.S.H.N., Mehta, Farhad F., Trivedi, Piyush
Formato:	Articulo Comunicacion
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia Plasma Cromatografía líquida human plasma; HPLC; validation; citicoline monosodium Agentes del Sistema Nervioso Central
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8217 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_2_4.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Plasma Cromatografía líquida human plasma; HPLC; validation; citicoline monosodium Agentes del Sistema Nervioso Central
spellingShingle	Farmacia Plasma Cromatografía líquida human plasma; HPLC; validation; citicoline monosodium Agentes del Sistema Nervioso Central Bindaiya, Shailendra K. Sahu, Kapendra Bhaisare, Mukesh Karthikeyan, Chandrabose Moorthy, N.S.H.N. Mehta, Farhad F. Trivedi, Piyush Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma
topic_facet	Farmacia Plasma Cromatografía líquida human plasma; HPLC; validation; citicoline monosodium Agentes del Sistema Nervioso Central
description	A sensitive and specific high performance reversed phase liquid chromatographic method was developed for quantification of citicoline monosodium (CTM) in human plasma. The active drug was isocratically eluted at a flow rate of 1 ml/min at ambient temperature in a nucleosil C18 analytical column with a mobile phase composed of tetrabutyl ammonium hydro gen sulfate buffer (0.005 M, pH5.0): methanol (95:05, v/v). Photodiode array (PDA) was performed at 270 nm and the retention time of the drug was found to be 6.64 min. The lowest limit of quantification (LLOQ) and of detection (LOD) were found to be 30 and 10 ng/ml, respectively. The method was validated and the response was found to be linear in the drug (CTM in spiked plasma) concentration range 150-900 ng/ml. The method was found to be accurate, with ranging from 96.38 to 98.65 % and precise, with intra-day, inter-day as well as analyst-toanalyst precision. The total recoveries of the method ranged between 95.69 and 97.89 %. Stability data revealed that the drug is stable in human plasma under various test conditions and the method can be successfully used for analysis of CTM in human plasma and in pharmacokinetic studies.
format	Articulo Comunicacion
author	Bindaiya, Shailendra K. Sahu, Kapendra Bhaisare, Mukesh Karthikeyan, Chandrabose Moorthy, N.S.H.N. Mehta, Farhad F. Trivedi, Piyush
author_facet	Bindaiya, Shailendra K. Sahu, Kapendra Bhaisare, Mukesh Karthikeyan, Chandrabose Moorthy, N.S.H.N. Mehta, Farhad F. Trivedi, Piyush
author_sort	Bindaiya, Shailendra K.
title	Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma
title_short	Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma
title_full	Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma
title_fullStr	Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma
title_full_unstemmed	Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma
title_sort	development and validation of a rp-hplc method for determination of citicoline monosodium in human plasma
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8217 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_2_4.pdf
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bdutipo_str	Repositorios
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Development and validation of a RP-HPLC method for determination of citicoline monosodium in human plasma

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