IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function

In rats, learning and memory performance decline during normal aging, which makes this rodent species a suitable model to evaluate therapeutic strategies. In aging rats, insulin-like growth factor-I (IGF-I), is known to significantly improve spatial memory accuracy as compared to control counterpart...

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Detalles Bibliográficos
Autores principales: Pardo, Joaquín, Abba, Martín Carlos, Lacunza, Ezequiel, Ogundele, Olalekan M., Paiva, Isabel, Morel, Gustavo Ramón, Outeiro, Tiago F., Goya, Rodolfo Gustavo
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Médicas
Aging
IGF-I
Spatial memory
Hippocampal transcriptome
Barnes maze
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82110
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82110
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Aging
IGF-I
Spatial memory
Hippocampal transcriptome
Barnes maze
spellingShingle Ciencias Médicas
Aging
IGF-I
Spatial memory
Hippocampal transcriptome
Barnes maze
Pardo, Joaquín
Abba, Martín Carlos
Lacunza, Ezequiel
Ogundele, Olalekan M.
Paiva, Isabel
Morel, Gustavo Ramón
Outeiro, Tiago F.
Goya, Rodolfo Gustavo
IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function
topic_facet Ciencias Médicas
Aging
IGF-I
Spatial memory
Hippocampal transcriptome
Barnes maze
description In rats, learning and memory performance decline during normal aging, which makes this rodent species a suitable model to evaluate therapeutic strategies. In aging rats, insulin-like growth factor-I (IGF-I), is known to significantly improve spatial memory accuracy as compared to control counterparts. A constellation of gene expression changes underlie the hippocampal phenotype of aging but no studies on the effects of IGF-I on the hippocampal transcriptome of old rodents have been documented. Here, we assessed the effects of IGF-I gene therapy on spatial memory performance in old female rats and compared them with changes in the hippocampal transcriptome. In the Barnes maze test, experimental rats showed a significantly higher exploratory frequency of the goal hole than controls. Hippocampal RNA-sequencing showed that 219 genes are differentially expressed in 28 months old rats intracerebroventricularly injected with an adenovector expressing rat IGF-I as compared with placebo adenovector-injected counterparts. From the differentially expressed genes, 81 were down and 138 upregulated. From those genes, a list of functionally relevant genes, concerning hippocampal IGF-I expression, synaptic plasticity as well as neuronal function was identified. Our results provide an initial glimpse at the molecular mechanisms underlying the neuroprotective actions of IGF-I in the aging brain.
format Articulo
Preprint
author Pardo, Joaquín
Abba, Martín Carlos
Lacunza, Ezequiel
Ogundele, Olalekan M.
Paiva, Isabel
Morel, Gustavo Ramón
Outeiro, Tiago F.
Goya, Rodolfo Gustavo
author_facet Pardo, Joaquín
Abba, Martín Carlos
Lacunza, Ezequiel
Ogundele, Olalekan M.
Paiva, Isabel
Morel, Gustavo Ramón
Outeiro, Tiago F.
Goya, Rodolfo Gustavo
author_sort Pardo, Joaquín
title IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function
title_short IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function
title_full IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function
title_fullStr IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function
title_full_unstemmed IGF-I Gene Therapy in Aging Rats Modulates Hippocampal Genes Relevant to Memory Function
title_sort igf-i gene therapy in aging rats modulates hippocampal genes relevant to memory function
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/82110
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