Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
Choline citrate (CC) and acetylmethionine (AM) are lipotropic drugs used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for simultaneous determination of CC and AM in injectable solutions, a...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8205 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_13.pdf |
| Aporte de: |
| Sumario: | Choline citrate (CC) and acetylmethionine (AM) are lipotropic drugs used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid
chromatographic (HPLC) method for simultaneous determination of CC and AM in injectable solutions,
aiming its application in routine analysis for quality control of these pharmaceutical formulations. The
method was validated using a Shim-Pack® C18 (250 x 4.6 mm, 5 µm) column. The mobile phase was constituted of 25 mM potassium phosphate buffer solution, pH 5.7, adjusted with 10 % orthophosphoric acid,
acetonitrile and methanol (88:10:2, v/v/v). The flow rate was 1.1 mL.min<sup>-1</sup>
and the UV detection was made
at 210 nm. The analyses were made at room temperature (25 ± 1 ºC). The method is precise, selective, accurate and robust, and was successfully applied for simultaneous quantitative determination of CC and
AM in injectables. |
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