Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography

Choline citrate (CC) and acetylmethionine (AM) are lipotropic drugs used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for simultaneous determination of CC and AM in injectable solutions, a...

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Autores principales: Alexandre, Grazielle P., Prado, Maria S. A., Kedor-Hackmann, Erika R.M., Singh, Anil K., Santoro, Maria Ines Rocha Miritello
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
acetylmethionine; Choline citrate; HPLC; injectable solutions; method validation
Agentes Lipotrópicos
Técnicas de Química Analítica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8205
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_13.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8205
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
acetylmethionine; Choline citrate; HPLC; injectable solutions; method validation
Agentes Lipotrópicos
Técnicas de Química Analítica
spellingShingle Farmacia
acetylmethionine; Choline citrate; HPLC; injectable solutions; method validation
Agentes Lipotrópicos
Técnicas de Química Analítica
Alexandre, Grazielle P.
Prado, Maria S. A.
Kedor-Hackmann, Erika R.M.
Singh, Anil K.
Santoro, Maria Ines Rocha Miritello
Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
topic_facet Farmacia
acetylmethionine; Choline citrate; HPLC; injectable solutions; method validation
Agentes Lipotrópicos
Técnicas de Química Analítica
description Choline citrate (CC) and acetylmethionine (AM) are lipotropic drugs used in several pharmaceutical formulations. The objective of this research was to develop and validate a high performance liquid chromatographic (HPLC) method for simultaneous determination of CC and AM in injectable solutions, aiming its application in routine analysis for quality control of these pharmaceutical formulations. The method was validated using a Shim-Pack® C18 (250 x 4.6 mm, 5 µm) column. The mobile phase was constituted of 25 mM potassium phosphate buffer solution, pH 5.7, adjusted with 10 % orthophosphoric acid, acetonitrile and methanol (88:10:2, v/v/v). The flow rate was 1.1 mL.min<sup>-1</sup> and the UV detection was made at 210 nm. The analyses were made at room temperature (25 ± 1 ºC). The method is precise, selective, accurate and robust, and was successfully applied for simultaneous quantitative determination of CC and AM in injectables.
format Articulo
Articulo
author Alexandre, Grazielle P.
Prado, Maria S. A.
Kedor-Hackmann, Erika R.M.
Singh, Anil K.
Santoro, Maria Ines Rocha Miritello
author_facet Alexandre, Grazielle P.
Prado, Maria S. A.
Kedor-Hackmann, Erika R.M.
Singh, Anil K.
Santoro, Maria Ines Rocha Miritello
author_sort Alexandre, Grazielle P.
title Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
title_short Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
title_full Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
title_fullStr Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
title_full_unstemmed Simultaneous determination of Choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
title_sort simultaneous determination of choline citrate and acetylmethionine in injectable solutions by high performance liquid chromatography
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8205
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_13.pdf
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