Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study
A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS) method for determination of troxerutin in rabbit plasma was developed. After addition of paeoniflorin as internal standard (IS), protein precipitation by methanol: acetonitrile (3:1, v/v) was used as sample pre...
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Autores principales: | , , , , , , , |
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Formato: | Articulo Comunicacion |
Lenguaje: | Inglés |
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2011
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8190 http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_2_16.pdf |
Aporte de: |
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I19-R120-10915-8190 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Farmacia LC-ESI-MS; rabbit plasma; troxerutin Técnicas de Química Analítica Flavonoles |
spellingShingle |
Farmacia LC-ESI-MS; rabbit plasma; troxerutin Técnicas de Química Analítica Flavonoles Ma Jianshe Zhang, Yuqing Zhang, Meiling Fan, Xiaofang Shentu, Yangping Wang, Xianqin Wang, Zhibin Wang, Zhiyi Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study |
topic_facet |
Farmacia LC-ESI-MS; rabbit plasma; troxerutin Técnicas de Química Analítica Flavonoles |
description |
A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS) method for determination of troxerutin in rabbit plasma was developed. After addition of paeoniflorin as internal standard (IS), protein precipitation by methanol: acetonitrile (3:1, v/v) was used as sample preparation. Chromatographic separation was achieved on an Allure (TM) PFP Propyl (2.1 mm × 100 mm, 5 μm) column with methanol-water as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantification using target fragment ions m/z 765 for troxerutin and m/z 503 for the IS. Calibration plots were linear over the range of 10-5000 ng/mL for troxerutin in rabbit plasma. Lower limit of quantitation (LLOQ) for troxerutin was 10 ng/mL. Mean recovery of troxerutin from plasma was in the range 92.6-98.1 %. RSD of intra-day and inter-day precision were both less than 11 %. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of troxerutin in rabbit plasma. |
format |
Articulo Comunicacion |
author |
Ma Jianshe Zhang, Yuqing Zhang, Meiling Fan, Xiaofang Shentu, Yangping Wang, Xianqin Wang, Zhibin Wang, Zhiyi |
author_facet |
Ma Jianshe Zhang, Yuqing Zhang, Meiling Fan, Xiaofang Shentu, Yangping Wang, Xianqin Wang, Zhibin Wang, Zhiyi |
author_sort |
Ma Jianshe |
title |
Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study |
title_short |
Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study |
title_full |
Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study |
title_fullStr |
Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study |
title_full_unstemmed |
Determination of troxerutin in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study |
title_sort |
determination of troxerutin in rabbit plasma by lc-esi-ms and its application to a pharmacokinetic study |
publishDate |
2011 |
url |
http://sedici.unlp.edu.ar/handle/10915/8190 http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_2_16.pdf |
work_keys_str_mv |
AT majianshe determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT zhangyuqing determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT zhangmeiling determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT fanxiaofang determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT shentuyangping determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT wangxianqin determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT wangzhibin determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy AT wangzhiyi determinationoftroxerutininrabbitplasmabylcesimsanditsapplicationtoapharmacokineticstudy |
bdutipo_str |
Repositorios |
_version_ |
1764820488507359233 |