Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column

A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS) method for determination of gemcitabine in rabbit plasma was developed. Chromatographic separation was achieved on a Restek Allure (TM) PFP Propyl (2.1 mm × 100 mm, 5 µm) column with (85: 15, v/v) methanol-wate...

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Detalles Bibliográficos
Autores principales: Lin, Guanyang, Hu, Lufeng, Pan, Yibin, Wang, Xianqin
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Técnicas de Química Analítica
Plasma
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8182
http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_2_7.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8182
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Técnicas de Química Analítica
Plasma
spellingShingle Farmacia
Técnicas de Química Analítica
Plasma
Lin, Guanyang
Hu, Lufeng
Pan, Yibin
Wang, Xianqin
Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column
topic_facet Farmacia
Técnicas de Química Analítica
Plasma
description A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS) method for determination of gemcitabine in rabbit plasma was developed. Chromatographic separation was achieved on a Restek Allure (TM) PFP Propyl (2.1 mm × 100 mm, 5 µm) column with (85: 15, v/v) methanol-water as mobile phase. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to quantify gemcitabine using target fragment ions m/z 264. Calibration plots were linear over the range of 5-4000 ng/mL for gemcitabine in plasma. Lower limit of quantitation (LLOQ) for gemcitabine was 5 ng/mL. Mean recovery of gemcitabine from plasma was in the range 91.0-95.5 %. RSD of intra-day and inter-day precision were less than 10 %, respectively. This method is simple, sensitive and fast enough to be used in pharmacokinetic research for determination of gemcitabine in rabbit plasma.
format Articulo
Comunicacion
author Lin, Guanyang
Hu, Lufeng
Pan, Yibin
Wang, Xianqin
author_facet Lin, Guanyang
Hu, Lufeng
Pan, Yibin
Wang, Xianqin
author_sort Lin, Guanyang
title Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column
title_short Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column
title_full Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column
title_fullStr Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column
title_full_unstemmed Determination of gemcitabine in rabbit plasma by LC-ESI-MS using an Allure PFP propyl column
title_sort determination of gemcitabine in rabbit plasma by lc-esi-ms using an allure pfp propyl column
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8182
http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_2_7.pdf
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AT hulufeng determinationofgemcitabineinrabbitplasmabylcesimsusinganallurepfppropylcolumn
AT panyibin determinationofgemcitabineinrabbitplasmabylcesimsusinganallurepfppropylcolumn
AT wangxianqin determinationofgemcitabineinrabbitplasmabylcesimsusinganallurepfppropylcolumn
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