Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry

The purpose of this study was to develop and validate analytical methods for determination of amlodipine besylate in tablets. Simple, accurate and precise liquid chromatographic and spectrophotometric methods are proposed. For the chromatographic method, the conditions were: a LiChrospher®100 RP-18...

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Autores principales: Leite, Helen D., Santoro, Maria Ines Rocha Miritello, Porto, João M.A., López García, Pedro, Almeida, Mariana M. de, Tavares, Vanessa F., Kedor-Hackmann, Erika R.M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
amlodipine besylate; chromatographic method; methods validation; spectrophotometric method
Técnicas de Química Analítica
Piridinas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8173
http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_1_17.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8173
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
amlodipine besylate; chromatographic method; methods validation; spectrophotometric method
Técnicas de Química Analítica
Piridinas
spellingShingle Farmacia
amlodipine besylate; chromatographic method; methods validation; spectrophotometric method
Técnicas de Química Analítica
Piridinas
Leite, Helen D.
Santoro, Maria Ines Rocha Miritello
Porto, João M.A.
López García, Pedro
Almeida, Mariana M. de
Tavares, Vanessa F.
Kedor-Hackmann, Erika R.M.
Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry
topic_facet Farmacia
amlodipine besylate; chromatographic method; methods validation; spectrophotometric method
Técnicas de Química Analítica
Piridinas
description The purpose of this study was to develop and validate analytical methods for determination of amlodipine besylate in tablets. Simple, accurate and precise liquid chromatographic and spectrophotometric methods are proposed. For the chromatographic method, the conditions were: a LiChrospher®100 RP-18 Merck® (125 mm x 4.6 mm, 5 µm) column; methanol/water containing 1 % of trietylamine adjusted to pH 5.0 with phosphoric acid (35:65) as mobile phase; a flow rate of 1.0 mL/min and UV detector at 238 nm. Linearity was in the range of 50.0 - 350.0 µg/mL with a correlation coefficient (r) = 0.9999. For the spectrophotometric method, the first dilutions of samples were performed in methanol and the consecutives in ultrapure water. The quantitation was made at 364.4 nm. Linearity was determined within the range of 41.0 - 61.0 µg/mL with a correlation coefficient (r) = 0.9996. Our results demonstrate that both methods can be used in routine analysis for quality control of tablets containing amlodipine besylate.
format Articulo
Articulo
author Leite, Helen D.
Santoro, Maria Ines Rocha Miritello
Porto, João M.A.
López García, Pedro
Almeida, Mariana M. de
Tavares, Vanessa F.
Kedor-Hackmann, Erika R.M.
author_facet Leite, Helen D.
Santoro, Maria Ines Rocha Miritello
Porto, João M.A.
López García, Pedro
Almeida, Mariana M. de
Tavares, Vanessa F.
Kedor-Hackmann, Erika R.M.
author_sort Leite, Helen D.
title Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry
title_short Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry
title_full Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry
title_fullStr Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry
title_full_unstemmed Quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and UV spectrophotometry
title_sort quantitative determination of amlodipine besylate in tablets by high performance liquid chromatography and uv spectrophotometry
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8173
http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_1_17.pdf
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