Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains

The aim of the present research was to study the optimisation of mevastatin production by Penicillium citrinum MTCC 1256. Optimization of fermentation medium was carried out by response surface methodology. Simultaneous estimation of mevastatin and citrinin was carried out by high performance thin l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ahmad, Ajaz, Mujeeb, Mohd, Panda, Bibhu P.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia Actinomedura sp.; citrinin; mevastatin; optimization; Penicillium citrinum Hongos Técnicas de Química Analítica
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8168 http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_1_12.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8168
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Actinomedura sp.; citrinin; mevastatin; optimization; Penicillium citrinum Hongos Técnicas de Química Analítica
spellingShingle	Farmacia Actinomedura sp.; citrinin; mevastatin; optimization; Penicillium citrinum Hongos Técnicas de Química Analítica Ahmad, Ajaz Mujeeb, Mohd Panda, Bibhu P. Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains
topic_facet	Farmacia Actinomedura sp.; citrinin; mevastatin; optimization; Penicillium citrinum Hongos Técnicas de Química Analítica
description	The aim of the present research was to study the optimisation of mevastatin production by Penicillium citrinum MTCC 1256. Optimization of fermentation medium was carried out by response surface methodology. Simultaneous estimation of mevastatin and citrinin was carried out by high performance thin layer chromatography. Glycerol and peptone were shown to be the best carbon and nitrogen source for mevastatin production by P. citrinum. Under optimized culture medium containing glycerol 10.94 g.l<sup>-1</sup> , peptone 8.32 g.l<sup>-1</sup> , CaCl<sub>2</sub> 0.53 g.l<sup>-1</sup> , MgSO<sub>4</sub> 0.52 g.l<sup>-1</sup> and KH<sub>2</sub>PO<sub>4</sub> 0.049 g.l<sup>-1</sup> resulted in a maximal mevastatin production of 522.5 mg.l<sup>-1</sup> Growth inhibited effect of citrinin on actinomycetes was . measured in terms of colony forming unit (CFU). There is a decrease in CFU of Actinomadura madura and Actinomadura livida with the increase in citrinin concentration. In terms of citrinin production, an 8-day fermentation would be preferable to a 14-day period, for the eventual bioconversion of mevastatin to pravastatin, since under these conditions spent broth would be free of this inhibitory product.
format	Articulo Articulo
author	Ahmad, Ajaz Mujeeb, Mohd Panda, Bibhu P.
author_facet	Ahmad, Ajaz Mujeeb, Mohd Panda, Bibhu P.
author_sort	Ahmad, Ajaz
title	Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains
title_short	Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains
title_full	Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains
title_fullStr	Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains
title_full_unstemmed	Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains
title_sort	production and optimization of mevastatin by penicillium citrinum mtcc 1256 and effect of citrinin on growth of actinomadura strains
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8168 http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_1_12.pdf
work_keys_str_mv	AT ahmadajaz productionandoptimizationofmevastatinbypenicilliumcitrinummtcc1256andeffectofcitrininongrowthofactinomadurastrains AT mujeebmohd productionandoptimizationofmevastatinbypenicilliumcitrinummtcc1256andeffectofcitrininongrowthofactinomadurastrains AT pandabibhup productionandoptimizationofmevastatinbypenicilliumcitrinummtcc1256andeffectofcitrininongrowthofactinomadurastrains
bdutipo_str	Repositorios
_version_	1764820488315469824

Production and optimization of mevastatin by Penicillium citrinum MTCC 1256 and effect of citrinin on growth of Actinomadura strains

Ejemplares similares