Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)

Ethanol and dichloromethane extracts from the marine sponge Iotrochota birotulata collected in the Urabá Gulf (Colombia) were tested against three human tumor cellular lines, including lung (A- 549), colon (HT29) and breast (MDA-MB-231), at 1 µg/mL, 5 &microg/mL and 25 &microg/mL. The most...

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Autores principales: Márquez, Diana M., Márquez Fernández, María Elena, Martínez, Alejandro, Thomas, Olivier P.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
bromotyrosine; Iotrochota birotulata; marine sponge
Extractos Vegetales
Línea Celular Tumoral
Técnicas y Procedimientos de Laboratorio
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8152
http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_2_9.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8152
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
bromotyrosine; Iotrochota birotulata; marine sponge
Extractos Vegetales
Línea Celular Tumoral
Técnicas y Procedimientos de Laboratorio
spellingShingle Farmacia
bromotyrosine; Iotrochota birotulata; marine sponge
Extractos Vegetales
Línea Celular Tumoral
Técnicas y Procedimientos de Laboratorio
Márquez, Diana M.
Márquez Fernández, María Elena
Martínez, Alejandro
Thomas, Olivier P.
Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)
topic_facet Farmacia
bromotyrosine; Iotrochota birotulata; marine sponge
Extractos Vegetales
Línea Celular Tumoral
Técnicas y Procedimientos de Laboratorio
description Ethanol and dichloromethane extracts from the marine sponge Iotrochota birotulata collected in the Urabá Gulf (Colombia) were tested against three human tumor cellular lines, including lung (A- 549), colon (HT29) and breast (MDA-MB-231), at 1 µg/mL, 5 &microg/mL and 25 &microg/mL. The most bioactive extract (ethanol), was separated by flash column chromatography obtaining six fractions which biological activity was evaluated on the same cellular lines A-549, HT29 and MDA-MB-231, at 1&microg/mL, 5 &microg/mL and 25 &microg/mL. The bioassay results showed that both extracts and two of the fractions totally inhibited the growing of the cellular line A549 at 25 &microg/mL, while the most active fraction showed total inhibition of the growing of the three tumor cell lines on the three evaluated concentrations. The most active fraction was separated by reversed phase HPLC which allowed isolating and then characterizing by mono- and bidimensional NMR, a halogenated compound derived from the bromotyrosine.
format Articulo
Comunicacion
author Márquez, Diana M.
Márquez Fernández, María Elena
Martínez, Alejandro
Thomas, Olivier P.
author_facet Márquez, Diana M.
Márquez Fernández, María Elena
Martínez, Alejandro
Thomas, Olivier P.
author_sort Márquez, Diana M.
title Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)
title_short Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)
title_full Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)
title_fullStr Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)
title_full_unstemmed Isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge Iotrochota birotulata from the Urabá Gulf (Colombia)
title_sort isolation of a bromotyrosine derivative compound of a bioactive fraction from the marine sponge iotrochota birotulata from the urabá gulf (colombia)
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8152
http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_2_9.pdf
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