Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry
A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS) method for determination of tetramethylpyrazine in rat plasma was developed. After addition of phenacetin as internal standard, protein precipitation by acetonitrile was used as sample preparation. Chromatog...
| Autores principales: | , , , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8148 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_2_5.pdf |
| Aporte de: |
| id |
I19-R120-10915-8148 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia LC-ESI-MS; rat plasma; tetramethylpyrazine Técnicas de Química Analítica Farmacocinética |
| spellingShingle |
Farmacia LC-ESI-MS; rat plasma; tetramethylpyrazine Técnicas de Química Analítica Farmacocinética Yu, Xiaomin Xu, Ren-ai Li, Junwei Ma Jianshe Deng, Jie Wang, Xianqin Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| topic_facet |
Farmacia LC-ESI-MS; rat plasma; tetramethylpyrazine Técnicas de Química Analítica Farmacocinética |
| description |
A sensitive and selective liquid chromatography/electrospray mass spectrometry (LC-ESIMS)
method for determination of tetramethylpyrazine in rat plasma was developed. After addition of
phenacetin as internal standard, protein precipitation by acetonitrile was used as sample preparation.
Chromatographic separation was achieved on a Zorbax SB-C18 (2.1 mm×150 mm, 5 µm) column with
(40:60, v/v) acetonitrile-water containing 0.1 % formic acid as mobile phase. Electrospray ionization (ESI)
source was applied and operated in positive ion mode; selected ion monitoring (SIM) mode was used to
quantify tetramethylpyrazine using target fragment ions m/z 136.9 for tetramethylpyrazine and m/z 179.8
for the IS. Calibration plots were linear over the range of 20-4000 ng/mL for tetramethylpyrazine in plasma.
Lower limit of quantitation (LLOQ) for tetramethylpyrazine was 20 ng/mL. Mean recovery of tetramethylpyrazine
from plasma was in the range 95.4-97.2 %. RSD of intra-day and inter-day precision were
less than 9 %, respectively. This method is simple, sensitive and fast enough to be used in pharmacokinetic
research for determination of tetramethylpyrazine in rat plasma. |
| format |
Articulo Comunicacion |
| author |
Yu, Xiaomin Xu, Ren-ai Li, Junwei Ma Jianshe Deng, Jie Wang, Xianqin |
| author_facet |
Yu, Xiaomin Xu, Ren-ai Li, Junwei Ma Jianshe Deng, Jie Wang, Xianqin |
| author_sort |
Yu, Xiaomin |
| title |
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| title_short |
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| title_full |
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| title_fullStr |
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| title_full_unstemmed |
Determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| title_sort |
determination of tetramethylpyrazine in rat plasma by liquid chromatography/electrospray mass spectrometry |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8148 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_2_5.pdf |
| work_keys_str_mv |
AT yuxiaomin determinationoftetramethylpyrazineinratplasmabyliquidchromatographyelectrospraymassspectrometry AT xurenai determinationoftetramethylpyrazineinratplasmabyliquidchromatographyelectrospraymassspectrometry AT lijunwei determinationoftetramethylpyrazineinratplasmabyliquidchromatographyelectrospraymassspectrometry AT majianshe determinationoftetramethylpyrazineinratplasmabyliquidchromatographyelectrospraymassspectrometry AT dengjie determinationoftetramethylpyrazineinratplasmabyliquidchromatographyelectrospraymassspectrometry AT wangxianqin determinationoftetramethylpyrazineinratplasmabyliquidchromatographyelectrospraymassspectrometry |
| bdutipo_str |
Repositorios |
| _version_ |
1764820488136163329 |