RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines

A simple, specific, sensitive and validated RP-HPLC method for the simultaneous estimation of amlodipine (AMB) and valsartan (VAL) in marketed tablet formulation was developed. The analysis was carried out on a phenomenex C18 (250 x 4.6 mm, 5 µm) column using a mobile phase of 0.1% ortho phosphoric...

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Detalles Bibliográficos
Autores principales:	Bhaisare, Mukesh, Sahu, Kapendra, Karthikeyan, Chandrabose, Moorthy, N.S.H.N., Trivedi, Piyush
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia amlodipine besylate; valsartan; RP-HPLC; simultaneous estimation Técnicas de Análisis Combinación de Medicamentos
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8142 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_1_18.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia amlodipine besylate; valsartan; RP-HPLC; simultaneous estimation Técnicas de Análisis Combinación de Medicamentos
spellingShingle	Farmacia amlodipine besylate; valsartan; RP-HPLC; simultaneous estimation Técnicas de Análisis Combinación de Medicamentos Bhaisare, Mukesh Sahu, Kapendra Karthikeyan, Chandrabose Moorthy, N.S.H.N. Trivedi, Piyush RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines
topic_facet	Farmacia amlodipine besylate; valsartan; RP-HPLC; simultaneous estimation Técnicas de Análisis Combinación de Medicamentos
description	A simple, specific, sensitive and validated RP-HPLC method for the simultaneous estimation of amlodipine (AMB) and valsartan (VAL) in marketed tablet formulation was developed. The analysis was carried out on a phenomenex C18 (250 x 4.6 mm, 5 µm) column using a mobile phase of 0.1% ortho phosphoric acid solution: acetonitrile (35:65 v/v, pH 3.0). The flow rate of the mobile phase was adjusted to 1.0 ml/min and was detected at 238 nm. The retention time obtained from the analysis was 1.995 min and 4.910 min for AMB and VAL respectively. The developed method was validated as per ICH guidelines. In order to find out the linearity, the concentrations ranging 2-10 µg/ml for AMB and 64-320 µg/ml for VAL was used. The squared correlation co-efficient (r 2 value) derived from the equation for AMB and VAL was 0.9979 and 0.9994, respectively. The percentage recoveries calculated for AMB and VAL ranges from 96.93 to 99.63 %. The estimated drug in the tablet formulation was 100.13 % and 99.99 % for AMB and VAL respectively. The results of analysis shows that method can be used for the estimation of AMB and VAL in the tablet dosage form without further separation in the quality control laboratories.
format	Articulo Articulo
author	Bhaisare, Mukesh Sahu, Kapendra Karthikeyan, Chandrabose Moorthy, N.S.H.N. Trivedi, Piyush
author_facet	Bhaisare, Mukesh Sahu, Kapendra Karthikeyan, Chandrabose Moorthy, N.S.H.N. Trivedi, Piyush
author_sort	Bhaisare, Mukesh
title	RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines
title_short	RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines
title_full	RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines
title_fullStr	RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines
title_full_unstemmed	RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines
title_sort	rp-hplc method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ich guidelines
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8142 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_1_18.pdf
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bdutipo_str	Repositorios
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RP-HPLC method for simultaneous estimation of amlodipine and valsartan in tablet formulation and validation as per ICH guidelines

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