In vitro cytotoxicity of carbon nanoparticles against Hep G 32 cells
The aim of the present study was to evaluate the in vitro toxicity of two multi wall carbon nanotubes (MWCNT) on human hepatocytes (Hep G 32 cell lines). The toxic effects of carbon nanoparticles were analyzed after 48 h of incubation with Hep G 32 cells using MTT assay and also estimated the levels...
Guardado en:
| Autores principales: | , , , |
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| Formato: | Articulo Comunicacion |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8119 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_2_3.pdf |
| Aporte de: |
| id |
I19-R120-10915-8119 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia carbon nanotubes; cytotoxicity; in vitro, Hep G 32; MWCNT Nanopartículas Test de citotoxicidad |
| spellingShingle |
Farmacia carbon nanotubes; cytotoxicity; in vitro, Hep G 32; MWCNT Nanopartículas Test de citotoxicidad Anreddy, Rama N. R. Devarakonda, Krishna R. Vurimindi, Himabindu Yellu, Narsimha R. In vitro cytotoxicity of carbon nanoparticles against Hep G 32 cells |
| topic_facet |
Farmacia carbon nanotubes; cytotoxicity; in vitro, Hep G 32; MWCNT Nanopartículas Test de citotoxicidad |
| description |
The aim of the present study was to evaluate the in vitro toxicity of two multi wall carbon nanotubes (MWCNT) on human hepatocytes (Hep G 32 cell lines). The toxic effects of carbon nanoparticles were analyzed after 48 h of incubation with Hep G 32 cells using MTT assay and also estimated the levels of LDH (that is leakage into the media). The results of the LDH estimation demonstrated that exposure of multi wall carbon nanotubes to hepatocytes (Hep G 32) for 48 h resulted in concentration-dependent increase in LDH leakage and exhibited a significant (p < 0.05) cytotoxicity at 3-50 μg/ml. Incubation of carbon nanotubes with liver cells produced a dose dependent inhibition of growth of the cells. The TC<sub>50</sub> or IC<sub>50</sub> values (toxic concentration 50 i.e. concentration of particles inducing 50 %cell mortality) of two nanoparticles were found in the range of 36.99-37.15 μg/ml, which were less than that of quartz (known toxic agent, 39.85 μg/ml), indicating the toxic nature of carbon nanoparticles |
| format |
Articulo Comunicacion |
| author |
Anreddy, Rama N. R. Devarakonda, Krishna R. Vurimindi, Himabindu Yellu, Narsimha R. |
| author_facet |
Anreddy, Rama N. R. Devarakonda, Krishna R. Vurimindi, Himabindu Yellu, Narsimha R. |
| author_sort |
Anreddy, Rama N. R. |
| title |
In vitro cytotoxicity of carbon nanoparticles
against Hep G 32 cells |
| title_short |
In vitro cytotoxicity of carbon nanoparticles
against Hep G 32 cells |
| title_full |
In vitro cytotoxicity of carbon nanoparticles
against Hep G 32 cells |
| title_fullStr |
In vitro cytotoxicity of carbon nanoparticles
against Hep G 32 cells |
| title_full_unstemmed |
In vitro cytotoxicity of carbon nanoparticles
against Hep G 32 cells |
| title_sort |
in vitro cytotoxicity of carbon nanoparticles
against hep g 32 cells |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8119 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_2_3.pdf |
| work_keys_str_mv |
AT anreddyramanr invitrocytotoxicityofcarbonnanoparticlesagainsthepg32cells AT devarakondakrishnar invitrocytotoxicityofcarbonnanoparticlesagainsthepg32cells AT vurimindihimabindu invitrocytotoxicityofcarbonnanoparticlesagainsthepg32cells AT yellunarsimhar invitrocytotoxicityofcarbonnanoparticlesagainsthepg32cells |
| bdutipo_str |
Repositorios |
| _version_ |
1764820487778598913 |