Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells

Bone and cartilage regeneration can be improved by designing a functionalized biomaterial that includes bioactive drugs in a biocompatible and biodegradable scaffold. Based on our previous studies, we designed a vanadium-loaded collagen scaffold for osteochondral tissue engineering. Collagen-vanadiu...

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Detalles Bibliográficos
Autores principales: Cortizo, Ana María, Ruderman, Graciela, Mazzini, Flavia Noelia, Molinuevo, María Silvina, Mogilner, Inés G.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2016
Materias:
Biología
scaffolds
vanadium
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/81057
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-81057
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
scaffolds
vanadium
spellingShingle Biología
scaffolds
vanadium
Cortizo, Ana María
Ruderman, Graciela
Mazzini, Flavia Noelia
Molinuevo, María Silvina
Mogilner, Inés G.
Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells
topic_facet Biología
scaffolds
vanadium
description Bone and cartilage regeneration can be improved by designing a functionalized biomaterial that includes bioactive drugs in a biocompatible and biodegradable scaffold. Based on our previous studies, we designed a vanadium-loaded collagen scaffold for osteochondral tissue engineering. Collagen-vanadium loaded scaffolds were characterized by SEM, FTIR, and permeability studies. Rat bone marrow progenitor cells were plated on collagen or vanadium-loaded membranes to evaluate differences in cell attachment, growth and osteogenic or chondrocytic differentiation. The potential cytotoxicity of the scaffolds was assessed by theMTT assay and by evaluation of morphological changes in cultured RAW264.7 macrophages. Our results show that loading of VOAsc did not alter the grooved ordered structure of the collagen membrane although it increased membrane permeability, suggesting a more open structure.The VOAsc was released to the media, suggesting diffusion-controlled drug release. Vanadiumloaded membranes proved to be a better substratum than 0 for all evaluated aspects of BMPC biocompatibility (adhesion, growth, and osteoblastic and chondrocytic differentiation). In addition, there was no detectable effect of collagen or vanadium-loaded scaffolds on macrophage viability or cytotoxicity. Based on these findings, we have developed a new ordered collagen scaffold loaded with VOAsc that shows potential for osteochondral tissue engineering.
format Articulo
Articulo
author Cortizo, Ana María
Ruderman, Graciela
Mazzini, Flavia Noelia
Molinuevo, María Silvina
Mogilner, Inés G.
author_facet Cortizo, Ana María
Ruderman, Graciela
Mazzini, Flavia Noelia
Molinuevo, María Silvina
Mogilner, Inés G.
author_sort Cortizo, Ana María
title Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells
title_short Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells
title_full Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells
title_fullStr Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells
title_full_unstemmed Novel Vanadium-Loaded Ordered Collagen Scaffold Promotes Osteochondral Differentiation of Bone Marrow Progenitor Cells
title_sort novel vanadium-loaded ordered collagen scaffold promotes osteochondral differentiation of bone marrow progenitor cells
publishDate 2016
url http://sedici.unlp.edu.ar/handle/10915/81057
work_keys_str_mv AT cortizoanamaria novelvanadiumloadedorderedcollagenscaffoldpromotesosteochondraldifferentiationofbonemarrowprogenitorcells
AT rudermangraciela novelvanadiumloadedorderedcollagenscaffoldpromotesosteochondraldifferentiationofbonemarrowprogenitorcells
AT mazziniflavianoelia novelvanadiumloadedorderedcollagenscaffoldpromotesosteochondraldifferentiationofbonemarrowprogenitorcells
AT molinuevomariasilvina novelvanadiumloadedorderedcollagenscaffoldpromotesosteochondraldifferentiationofbonemarrowprogenitorcells
AT mogilnerinesg novelvanadiumloadedorderedcollagenscaffoldpromotesosteochondraldifferentiationofbonemarrowprogenitorcells
bdutipo_str Repositorios
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