Standardization of Caco-2 cell culture as in vitro model for intestinal permeability
The aim of this study was to find out the optimal experimental conditions for Caco-2 cell culture (time and density) and permeability assays (diffusion system and drug concentration) in order to study the in vitro drugs permeability as a predictive method for drug absorption across intestinal epit...
Guardado en:
| Autores principales: | , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8097 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_5.pdf |
| Aporte de: |
| id |
I19-R120-10915-8097 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Técnicas de Cultivo de Célula atenolol; biopharmaceutics classification system; Caco-2; permeability Permeabilidad |
| spellingShingle |
Farmacia Técnicas de Cultivo de Célula atenolol; biopharmaceutics classification system; Caco-2; permeability Permeabilidad Rodríguez, Yanina I. Abalos, Ivana S. Lozano, Verónica Cereseto, Marina Silberman, Nicolás G. Chiale, Carlos Gruñeiro, Elena Pesce, Guido Standardization of Caco-2 cell culture as in vitro model for intestinal permeability |
| topic_facet |
Farmacia Técnicas de Cultivo de Célula atenolol; biopharmaceutics classification system; Caco-2; permeability Permeabilidad |
| description |
The aim of this study was to find out the optimal experimental conditions for Caco-2 cell culture
(time and density) and permeability assays (diffusion system and drug concentration) in order to
study the in vitro drugs permeability as a predictive method for drug absorption across intestinal epithelium.
The integrity of the monolayers used in each assay was determined by measuring the transepithelial
electrical resistance (TEER) and the permeability of the atenolol-a drug which is transported across the
monolayers by the paracellular pathway-. The best working condition was obtained with a cell seeding of
7.10<sup>4</sup> cells/insert in a vertical difussion chamber. In such context, the monolayers had a TEER higher than
550 Ω.cm<sup>2</sup> and the apparent permeability coefficient of atenolol was 0.71 ± 0.19 x 10<sup>-6</sup> cm/seg. |
| format |
Articulo Articulo |
| author |
Rodríguez, Yanina I. Abalos, Ivana S. Lozano, Verónica Cereseto, Marina Silberman, Nicolás G. Chiale, Carlos Gruñeiro, Elena Pesce, Guido |
| author_facet |
Rodríguez, Yanina I. Abalos, Ivana S. Lozano, Verónica Cereseto, Marina Silberman, Nicolás G. Chiale, Carlos Gruñeiro, Elena Pesce, Guido |
| author_sort |
Rodríguez, Yanina I. |
| title |
Standardization of Caco-2 cell culture
as in vitro model for intestinal permeability |
| title_short |
Standardization of Caco-2 cell culture
as in vitro model for intestinal permeability |
| title_full |
Standardization of Caco-2 cell culture
as in vitro model for intestinal permeability |
| title_fullStr |
Standardization of Caco-2 cell culture
as in vitro model for intestinal permeability |
| title_full_unstemmed |
Standardization of Caco-2 cell culture
as in vitro model for intestinal permeability |
| title_sort |
standardization of caco-2 cell culture
as in vitro model for intestinal permeability |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8097 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_5.pdf |
| work_keys_str_mv |
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| bdutipo_str |
Repositorios |
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1764820487949516802 |