Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion hydrochloride in rat plasma using triazolam as an internal standard. Chromatographic separation was achieved on a SB-C18 column at 30 °C, with 50: 50 (v/v) aceton...
Guardado en:
| Autores principales: | , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8094 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_2.pdf |
| Aporte de: |
| id |
I19-R120-10915-8094 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas |
| spellingShingle |
Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas Hu, Lufeng Lin, Guanyang Wang, Xianqin Xu, Ren-ai Zhang, Xiuhua Zhang, Chunhong Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study |
| topic_facet |
Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas |
| description |
A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion hydrochloride in rat plasma using triazolam as an internal standard. Chromatographic separation was achieved on a SB-C18 column at 30 °C, with 50: 50 (v/v) acetonitrile-0.1 % formic acid in water as mobile phase. The flow rate was 0.3 mL/min. The determination of bupropion was performed in MRM mode, m/z 239.9 → 183.7 for bupropion and m/z 343.0 → 308.0 for triazolam (IS) and positive ion electrospray ionization interface. Calibration curve was linear over range of 1.2 to 480 ng/mL. The intra- and inter-run relative standard deviations of the assay were less than 10 %. The mean absolute recoveries determined at the concentrations of 2.4, 48 and 360 ng/mLwere 91.00%, 92.06%, 91.71%, respectively. The validated method is successfully used to analyze the drug in samples of rat plasma for pharmacokinetic study. |
| format |
Articulo Articulo |
| author |
Hu, Lufeng Lin, Guanyang Wang, Xianqin Xu, Ren-ai Zhang, Xiuhua Zhang, Chunhong |
| author_facet |
Hu, Lufeng Lin, Guanyang Wang, Xianqin Xu, Ren-ai Zhang, Xiuhua Zhang, Chunhong |
| author_sort |
Hu, Lufeng |
| title |
Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study |
| title_short |
Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study |
| title_full |
Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study |
| title_fullStr |
Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study |
| title_full_unstemmed |
Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study |
| title_sort |
determination of bupropion hydrochloride in rat plasma by lc-ms/ms and its application to pharmacokinetic study |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8094 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_2.pdf |
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AT hulufeng determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT linguanyang determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT wangxianqin determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT xurenai determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT zhangxiuhua determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT zhangchunhong determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy |
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Repositorios |
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1764820487899185152 |