Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation

Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts r...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fantinelli, Juliana Catalina, Cuéllar Álvarez, Liceth N., González Arbeláez, Luisa Fernanda, Ciocci Pardo, Alejandro, Galeano García, Paula, Schinella, Guillermo Raúl, Mosca, Susana María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/80930
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-80930
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
spellingShingle Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula
Schinella, Guillermo Raúl
Mosca, Susana María
Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
topic_facet Ciencias Médicas
Copoazú
Fermentation
Ischemia-reperfusion
NOS
Mitochondria
description Our aim was to examine the effects of aqueous extracts of fermented (CFE) and non-fermented (CNFE) ¨copoazú¨ (fruit of Theobroma grandiflorum) seeds against ischemia-reperfusion injury. Isolated rat hearts were submitted to 30 min of global ischemia (GI) and 60 min of reperfusion (R). Other hearts received CFE or CNFE in absence or in presence of L-NAME (a nitric oxide synthase inhibitor). Infarct size (IS) and postischemic myocardial function (PMF) were measured. Lipid peroxidation, reduced glutathione (GSH) and the expression of phosphorylated forms of eNOS, Akt, GSK-3b and PKCe were assessed. The response of isolated mitochondria to Ca2+ (MR) was also determined. CFE but not CNFE decreased IS, increased PMF and the expression of P-eNOS, P-Akt, P-GSK-3b and P-PKCe, partially preserved GSH and improved MR. These effects were lost in presence of L-NAME. These data demonstrate that the acute treatment with CFE protects the heart against ischemiareperfusion damage through NOS-dependent mechanisms.
format Articulo
Articulo
author Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula
Schinella, Guillermo Raúl
Mosca, Susana María
author_facet Fantinelli, Juliana Catalina
Cuéllar Álvarez, Liceth N.
González Arbeláez, Luisa Fernanda
Ciocci Pardo, Alejandro
Galeano García, Paula
Schinella, Guillermo Raúl
Mosca, Susana María
author_sort Fantinelli, Juliana Catalina
title Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_short Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_full Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_fullStr Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_full_unstemmed Acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via eNOS activation
title_sort acute treatment with copoazú fermented extract ameliorates myocardial ischemia-reperfusion injury via enos activation
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/80930
work_keys_str_mv AT fantinellijulianacatalina acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
AT cuellaralvarezlicethn acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
AT gonzalezarbelaezluisafernanda acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
AT cioccipardoalejandro acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
AT galeanogarciapaula acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
AT schinellaguillermoraul acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
AT moscasusanamaria acutetreatmentwithcopoazufermentedextractamelioratesmyocardialischemiareperfusioninjuryviaenosactivation
bdutipo_str Repositorios
_version_ 1764820487889747968

Ejemplares similares