Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest

The object of the present study was to investigate the anticancer properties of total alkaloids from Solanum lyratum Thunb (SLT-A), including the inhibitory effect of SLT-A on HeLa cells and the apoptosis-inducing capacity in vitro. In our study, cytotoxicity was measured by the growth inhibition...

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Detalles Bibliográficos
Autores principales: Lu, Wenzong, Ni, Yuan, Zhao, Chen, Zhang, Liang, Ren, Yumiao
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
alkaloids; anticancer; apoptosis; hela cells; solanum lyratum thunb.
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8079
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_19.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8079
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
alkaloids; anticancer; apoptosis; hela cells; solanum lyratum thunb.
spellingShingle Farmacia
alkaloids; anticancer; apoptosis; hela cells; solanum lyratum thunb.
Lu, Wenzong
Ni, Yuan
Zhao, Chen
Zhang, Liang
Ren, Yumiao
Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
topic_facet Farmacia
alkaloids; anticancer; apoptosis; hela cells; solanum lyratum thunb.
description The object of the present study was to investigate the anticancer properties of total alkaloids from Solanum lyratum Thunb (SLT-A), including the inhibitory effect of SLT-A on HeLa cells and the apoptosis-inducing capacity in vitro. In our study, cytotoxicity was measured by the growth inhibition assay and detection of apoptosis was performed by Hoechst33324 and Tdt-mediated dUTP nick end labeling (TUNEL) staining assays. The in vitro cytotoxic studies were complemented by the cell cycle analysis and determination caspase-3 activity. Reverse transcription-polymerase chain reaction (RT-PCR) assay was applied on the expression of apoptosis-associated genes. The result showed that treatment of HeLa cells with SLT-A resulted in the growth inhibition effect, and the IC<sub>50</sub> value was approximately 82 µg/ml. SLTA (80 µg/ml) induced more cell apoptosis of HeLa cells and accumulated the cells in the G2/M phase compared with the control cells. On the other hand, the expression of p53 and Bax gene was increased in the cells treated with SLT-A (80 µg/ml), with an increase in the activity of caspase-3, while Bcl-2 expression was not changed compared to the control cells. Our results demonstrated that SLT-A presented antiproliferative activity in HeLa cells and might be a potential anticancer drug.
format Articulo
Articulo
author Lu, Wenzong
Ni, Yuan
Zhao, Chen
Zhang, Liang
Ren, Yumiao
author_facet Lu, Wenzong
Ni, Yuan
Zhao, Chen
Zhang, Liang
Ren, Yumiao
author_sort Lu, Wenzong
title Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
title_short Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
title_full Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
title_fullStr Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
title_full_unstemmed Total alkaloids from Solanum Iyratum Thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
title_sort total alkaloids from solanum iyratum thunb. inhibited hela cells proliferation through induction of apoptosis and cell cycle arrest
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8079
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_19.pdf
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AT niyuan totalalkaloidsfromsolanumiyratumthunbinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
AT zhaochen totalalkaloidsfromsolanumiyratumthunbinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
AT zhangliang totalalkaloidsfromsolanumiyratumthunbinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
AT renyumiao totalalkaloidsfromsolanumiyratumthunbinhibitedhelacellsproliferationthroughinductionofapoptosisandcellcyclearrest
bdutipo_str Repositorios
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