Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column...
Guardado en:
| Autores principales: | , |
|---|---|
| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2010
|
| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8077 http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_17.pdf |
| Aporte de: |
| Sumario: | A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column (250 x 4.6, 5 µm). Diode array detection at a wavelength of 240 nm was carried out with a mobile phase comprising of a mixture of 0.1M ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of (45:55, v/v). The method was linear in the concentration range of 0.1-20 µg/ml for atorvastatin and 6-1200 µg/ml for gemfibrozil with correlation coefficient between 0.9997 and 0.9976. The limit of detection was 0.03 µg/ml for atorvastatin and 1.8 µg/ml for gemfibrozil. The limit of quantitation was 0.1 µg/ml for atorvastatin and 6 µg/ml for gemfibrozil. The average recovery of both the analytes was greater than 75 %. The method was validated in terms of linearity, recovery, precision, specificity, LOD/LOQ values and stability of solutions and it was applied successfully for the determination of atorvastatin and gemfibrozil in spiked human plasma. |
|---|