Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography

A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column...

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Detalles Bibliográficos
Autores principales: Khan, Islam U., Ashfaq, Muhammad
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
atorvastatin; diode array detector; gemfibrozil; HPLC; plasma
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8077
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_17.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8077
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
atorvastatin; diode array detector; gemfibrozil; HPLC; plasma
spellingShingle Farmacia
atorvastatin; diode array detector; gemfibrozil; HPLC; plasma
Khan, Islam U.
Ashfaq, Muhammad
Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
topic_facet Farmacia
atorvastatin; diode array detector; gemfibrozil; HPLC; plasma
description A simple, sensitive and reproducible RP-HPLC method was developed and validated for the simultaneous determination of atorvastatin and gemfibrozil in human plasma. After a single step liquidliquid extraction of both the drugs with acetonitrile, the separation was accomplished on a Merck C 18 column (250 x 4.6, 5 µm). Diode array detection at a wavelength of 240 nm was carried out with a mobile phase comprising of a mixture of 0.1M ammonium acetate buffer (pH 5.0) and acetonitrile in the ratio of (45:55, v/v). The method was linear in the concentration range of 0.1-20 µg/ml for atorvastatin and 6-1200 µg/ml for gemfibrozil with correlation coefficient between 0.9997 and 0.9976. The limit of detection was 0.03 µg/ml for atorvastatin and 1.8 µg/ml for gemfibrozil. The limit of quantitation was 0.1 µg/ml for atorvastatin and 6 µg/ml for gemfibrozil. The average recovery of both the analytes was greater than 75 %. The method was validated in terms of linearity, recovery, precision, specificity, LOD/LOQ values and stability of solutions and it was applied successfully for the determination of atorvastatin and gemfibrozil in spiked human plasma.
format Articulo
Articulo
author Khan, Islam U.
Ashfaq, Muhammad
author_facet Khan, Islam U.
Ashfaq, Muhammad
author_sort Khan, Islam U.
title Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
title_short Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
title_full Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
title_fullStr Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
title_full_unstemmed Determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
title_sort determination of atorvastatin and gemfibrozil in human plasma by reversed-phase liquid chromatography
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8077
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_17.pdf
work_keys_str_mv AT khanislamu determinationofatorvastatinandgemfibrozilinhumanplasmabyreversedphaseliquidchromatography
AT ashfaqmuhammad determinationofatorvastatinandgemfibrozilinhumanplasmabyreversedphaseliquidchromatography
bdutipo_str Repositorios
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