Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole

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Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to ev...

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Autores principales: López Nigro, Marcela M., Mudry, Marta Dolores, Carballo, Marta A.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
cell death; cytotoxicity; genetic toxicology; genotoxicity; metronidazole; short term tests
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8068
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_8.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8068
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
cell death; cytotoxicity; genetic toxicology; genotoxicity; metronidazole; short term tests
spellingShingle Farmacia
cell death; cytotoxicity; genetic toxicology; genotoxicity; metronidazole; short term tests
López Nigro, Marcela M.
Mudry, Marta Dolores
Carballo, Marta A.
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
topic_facet Farmacia
cell death; cytotoxicity; genetic toxicology; genotoxicity; metronidazole; short term tests
description Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.001), morphological evidence of apoptosis and increase in the percentage of nuclei with hypodiploid DNA content (P < 0.001). We concluded that MTZ is genotoxic, cytotoxic and is able to modulate cell death through apoptotic mechanisms in the experimental design employed.
format Articulo
Articulo
author López Nigro, Marcela M.
Mudry, Marta Dolores
Carballo, Marta A.
author_facet López Nigro, Marcela M.
Mudry, Marta Dolores
Carballo, Marta A.
author_sort López Nigro, Marcela M.
title Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
title_short Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
title_full Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
title_fullStr Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
title_full_unstemmed Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
title_sort use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8068
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_1_8.pdf
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bdutipo_str Repositorios
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